The most common "Blue" MCO enzymes are ascorbate oxidase
(AOx), laccase (Lac), and bilirubin oxidase (BOx) that can act as excellent biocathode materials for biofuel cell applications [5, 6].
One hundred [micro]L of vegetable aqueous extracts (5mg/mL) was mixed with 40 [micro]L of ascorbate oxidase
Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signaling.
Effect of ascorbate oxidase overexpression on ascorbate recycling gene expression in response to agents imposing oxidative stress.
Current studies have exposed the capability of flavonoids to influence transportation of vitamin C around the body, and also assist to control the role of an enzyme called ascorbate oxidase
, which converts vitamin C into non vitamin type.
Clove oil is also an important ingredient in weed killer and herbicides.A large number of metal containing proteins, such as hemocyanin (a Cu metal containingprotein present in some invertebrate animals which helps them in the transport of O2;like hemoglobin in humans) retain metal ions in their active sites so a great interest is developed in such metal containing enzymes which are ureases, laccase, tyrosinase and ascorbate oxidase
[5-9] containing enzyme urease (urea amidohydrolase EC 3.5.15) act as a catalyst to hydrolyse urea to ammonia and carbon dioxide.
Some of the antioxidant enzymes that are found to provide protection against the ROS are superoxide dismutase, catalase, peroxidase, glutathione peroxidase, and ascorbate oxidase
(Rani et al., 2004).
To prevent ascorbic acid from hindering the peroxide and its generated chromogen, we added ascorbate oxidase
to the reagent.
In regard to uric acid, it should be pointed out that vitamin C can interfere with the standard colorimetric uric acid assay, though excess ascorbate oxidase
is usually added during the assays to prevent this.
The ascorbate assay buffer consisted of 200 mM sodium acetate, pH 5.6; 100 [micro]l of pH-adjusted sample extract was mixed with 900 [micro]l assay buffer, and the change in absorbance at 265 nm was recorded after reaction with 2 units ascorbate oxidase
(Logan et al., 1998b).