The enzymes, such as hyaluronidase, phospholipase [A.sub.2] ([PLA.sub.2]), [alpha]-D-glucosidase, lysopphospholipase, [alpha]-acetylamino-deosiglucosidase, arylamidase
and phosphomonoesterase acid esterase.
Catalase tests, growth at 6.5% salt, bile esculin hydrolysis, and the pyrrolidonyl arylamidase
(PYR) test were used to identify the genera and strains.
dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase
, cystine arylamidase
, napthol-AS-BI-phosphohydrolase, [beta]-galactosidase, leucine arylamidase
, and trypsin.
For the purpose of this study, organisms were identified based on colonial morphology, Gram stain, and rapid spot tests such as catalase, Staphaurex[TM] Plus latex agglutination, pyrrolidonyl arylamidase
, Remel[TM] Microdase discs, indole, and oxidase (ThermoFisher Scientifics, Inc., Middletown, VA).
Biochemical reaction Result Nitrate + Growth in MacConkey + Urea hydrolysis + Esculin hydrolysis + Gelatin hydrolysis - ONPG (o-nitrophenyl-[beta]-D-galactopyranoside) + Oxidase + Vogesproskauer - [beta]-Galactosidase - Tryptophan deaminase - Ornithine decarboxylase - Lysine decarboxylase - Arginine decarboxylase - Motility + Glucose (assimilation) + Mannose + Mannitol + Maltose + Gluconate + Arabinose + Adonitol + Glutamyl arylamidase
pNA + Indole -
We found statistically significant correlations between increased resistance to 5-fluorocytosine and higher activity of acid phosphatase; increased resistance to ketoconazole and higher activity of beta-glucuronidase; increased resistance to miconazole and higher activity of beta-glucosidase; increased resistance to itraconazole and higher activity of N-acetyl-beta-D-glucosidase, as well as increased susceptibility to itraconazole and higher enzymatic activity of cystine arylamidase
and naphthyl phosphohydrolase.
Leuconostoc species are incapable of producing leucine aminopeptidase and pyrrolidonyl arylamidase
. [CO.sub.2] formation with glucose are distinctive properties while not a part of routine investigations in many microbiology labs.
Various biochemical tests were done with the suspected colony for final identification of Enterococci eg., catalase test, Pyrase (L-pyrrolidonyl arylamidase
) test, Bileesculin test, Salt-tolerance test with 6.5% NaCl broth, growth at 10[degrees]C and 45[degrees]C, growth at 9.6 pH, Heat tolerance test at 60[degrees]C for 30 minutes in a water bath.
With the APIZYM system, semiquantitative evaluation of the activities of 19 hydrolytic enzymes [alkaline phosphatase, esterase (C4), esterase-lipase (C8), lipase (C14), leucine arylamidase
, valine arylamidase
, cystine arylamidase
, trypsin, [alpha]-chymotrypsin, acid phosphatase, phosphoamidase, [alpha]-galactosidase, [beta]-galactosidase, [beta]-glucuronidase, [alpha]-glucosidase, [beta]-glucosidase, N-acetyl-[beta]-glucosaminidase, [alpha]-mannosidase, and [alpha]-fucosidase] was determined [51, 52].
Different from the other five Cellvibrio species, strain PR1 utilizes agar and carboxymethyl cellulose for the growth and shows [beta]-galactosidase and afucosidase activities, but leucine arylamidase
activity was not observed.
Alkaline phosphatase, acid phosphatase, leucine arylamidase
, and jV-acteyl-P-glucosaminidase (NAGase) were the only enzymes available for analysis.