The enzymes, such as hyaluronidase, phospholipase [A.sub.2] ([PLA.sub.2]), [alpha]-D-glucosidase, lysopphospholipase, [alpha]-acetylamino-deosiglucosidase, arylamidase
and phosphomonoesterase acid esterase.
Catalase tests, growth at 6.5% salt, bile esculin hydrolysis, and the pyrrolidonyl arylamidase
(PYR) test were used to identify the genera and strains.
dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase
, cystine arylamidase
, napthol-AS-BI-phosphohydrolase, [beta]-galactosidase, leucine arylamidase
, and trypsin.
For the purpose of this study, organisms were identified based on colonial morphology, Gram stain, and rapid spot tests such as catalase, Staphaurex[TM] Plus latex agglutination, pyrrolidonyl arylamidase
, Remel[TM] Microdase discs, indole, and oxidase (ThermoFisher Scientifics, Inc., Middletown, VA).
Biochemical reaction Result Nitrate + Growth in MacConkey + Urea hydrolysis + Esculin hydrolysis + Gelatin hydrolysis - ONPG (o-nitrophenyl-[beta]-D-galactopyranoside) + Oxidase + Vogesproskauer - [beta]-Galactosidase - Tryptophan deaminase - Ornithine decarboxylase - Lysine decarboxylase - Arginine decarboxylase - Motility + Glucose (assimilation) + Mannose + Mannitol + Maltose + Gluconate + Arabinose + Adonitol + Glutamyl arylamidase
pNA + Indole -
We found statistically significant correlations between increased resistance to 5-fluorocytosine and higher activity of acid phosphatase; increased resistance to ketoconazole and higher activity of beta-glucuronidase; increased resistance to miconazole and higher activity of beta-glucosidase; increased resistance to itraconazole and higher activity of N-acetyl-beta-D-glucosidase, as well as increased susceptibility to itraconazole and higher enzymatic activity of cystine arylamidase
and naphthyl phosphohydrolase.
Leuconostoc species are incapable of producing leucine aminopeptidase and pyrrolidonyl arylamidase
. [CO.sub.2] formation with glucose are distinctive properties while not a part of routine investigations in many microbiology labs.
Various biochemical tests were done with the suspected colony for final identification of Enterococci eg., catalase test, Pyrase (L-pyrrolidonyl arylamidase
) test, Bileesculin test, Salt-tolerance test with 6.5% NaCl broth, growth at 10[degrees]C and 45[degrees]C, growth at 9.6 pH, Heat tolerance test at 60[degrees]C for 30 minutes in a water bath.
With the APIZYM system, semiquantitative evaluation of the activities of 19 hydrolytic enzymes [alkaline phosphatase, esterase (C4), esterase-lipase (C8), lipase (C14), leucine arylamidase
, valine arylamidase
, cystine arylamidase
, trypsin, [alpha]-chymotrypsin, acid phosphatase, phosphoamidase, [alpha]-galactosidase, [beta]-galactosidase, [beta]-glucuronidase, [alpha]-glucosidase, [beta]-glucosidase, N-acetyl-[beta]-glucosaminidase, [alpha]-mannosidase, and [alpha]-fucosidase] was determined [51, 52].
iniae: acetoin production (Voges Proskauer) (Remel, USA), aminopeptidase leucine (LAP) (Remel, USA), arylamidase
pyrrolidonyl (PYR) (Remel, USA), starch hydrolysis (Merck, Germany) and esculin hydrolysis (Neogen, Acumedia, USA).
Different from the other five Cellvibrio species, strain PR1 utilizes agar and carboxymethyl cellulose for the growth and shows [beta]-galactosidase and afucosidase activities, but leucine arylamidase
activity was not observed.
Alkaline phosphatase, acid phosphatase, leucine arylamidase
, and jV-acteyl-P-glucosaminidase (NAGase) were the only enzymes available for analysis.