Phenobarbital and montelukast were dissolved in 0.5% dimethyl sulfoxide and sterile apyrogenic
saline containing 10% propylene glycol.
C57BL/6 mice were given subcutaneous injections of 0.1 mL of apyrogenic
saline (BioManguinhos, Fiocruz, Rio de Janeiro, RJ, Brazil) containing 10 [micro]g of anti-human TNF blocking antibody (Infliximab, Remicade), a gift from Schering-Plough of Brazil, 48-hour intervals from 120 to 150 days postinfection (dpi).
After administration of 1% tetracaine eye drops, the inferior eye lid was reverted with sterile gauze, and the follicles in the inferior conjunctiva were scraped with sterile, disposable, apyrogenic
, stainless-steel, Kimura-like spatulas.
Prevention of these responses can be obtained by using ultrapure dialysate or ultrapure water, which in the dialysis realm refers to the reduction of bacteria and its by-products (endotoxins) resulting in a pyrogen-free or apyrogenic
solution (see Sidebar).