Figure 1 shows all steps of
aptamer selection in this study.
(61) Moreover, blocking of CFIm25 by a specific RNA
aptamer produced alteration in the recruitment, cleavage and polyadenylation reactions.
Aptamer nano-flares for molecular detection in living cells.
Keywords: Cell-SELEX, DNA
aptamer. Exonucleases, Prostate specific membrane antigen
Recently, the same conjugated STX-KLH was used to select
aptamers in vitro, through the immobilization of the STX-KLH conjugate with an anti-KLH antibody which was previously conjugated with MB (Handy et al., 2013).
Incorporation of additional chemical groups with certain properties allows to considerably expand variation of a library, which increases the chances to select a specific
aptamer even for complex non-protein compounds, such as glycans.
Eccrine's successful development of
aptamer biosensors will have great value to the healthcare industry and eventually other markets.
According to the company,
aptamers are small, single-stranded DNA or RNA molecules that are identified and manufactured in vitro, which fold into shapes that fit their targets.
Aptamer Group is a UK-based biotechnology company developing
aptamers, nucleic acid molecules with affinity and selectivity for a specified target, as an alternative to antibodies.
To evaluate the impact of peptide on the binding affinity of the
aptamer, we also incubated the beads with the same concentrations of fluorescein isothiocyanate-labeled free
aptamer and the aptamer--peptide probe, respectively (see the Methods section in the online Data Supplement).
Field-effect-transistor- (FET-) like structures incorporating graphene substrates and DNA
aptamer molecular sensing elements have been used to detect small molecules and ions of physiological interest [10].
A new therapeutic approach against liver metastasis was developed through the identification of an antimetastatic, CEA-specific RNA
aptamer [70].
