HDL containing apoA-I and apoAII
helps to degrade cholesterol from VLDL and chylomicron by reverse cholesterol transport from peripheral tissue to liver .
The remainder is chiefly apoAi and apolipoprotein Aii (apoAii
Yu et al., "Expression of human ApoAII
in transgenic rabbits leads to dyslipidemia: a new model for combined hyperlipidemia," Arteriosclerosis, Thrombosis, and Vascular Biology, vol.
Differential tissue-specific expression of human apoA-I and apoAII
. J Lipid Res 1991;32:821-8.
Lipids and lipoproteins metabolism in humans may be controlled by genes, including the lipoprotein lipase, apo AI, apo E, apo B, apo C, apoAII
, and lecithin: cholesterol acyltransferase genes, because of their central position in the lipids metabolism regulationt (19,20).
They include apoAI, apoAII
, apoAIV, apoAV, apoCI, apoCII, apoCIII and apoE as well as several lesser-known apoproteins.
The annealing temperature was lowered to 58 [degrees]C for ApoD and ApoAII
genes and 55 [degrees]C for UCP3, UCP2, IRS-1, Ob-Rb, HSL, RxR, and LPL.
Compared to native LDL, it has an increased content of several non-apoB apolipoproteins: apoE, apoCIII, apoAI, apoAII
, apoD, apoF, and apoJ .
Micronized fenofibrate is a fibric acid derivative that reduces serum TC, LDL-C, TG, and VLDL-cholesterol and increases the concentrations of HDL-C, apoAI, and apoAII (22).
 Nonstandard abbreviations: IMT, intima-media thickness; TC, total cholesterol; LDL-C, LDL-cholesterol; TG, triglyceride; HDL-C, HDL-cholesterol; hsCRP, high-sensitivity C-reactive protein; apoB100, apolipoprotein B100; apoAI, apolipoprotein AI; IL-1, interleukin-1; IL-6, interleukin-6; IFN-[gamma], interferon [gamma]; TNF-[alpha], tumor necrosis factor; OxLDL, oxidized LDL; NO, nitric oxide; apoAII, apolipoprotein II; D, diameter; CCAIMT, common carotid artery IMT; ICAIMT, internal carotid artery IMT.
In addition to apoE, the CSF also contains apoAI, apoAII
, apoAVI, apoD, and apoJ (8-10) but not apoB (the only apolipoprotein included in the LDL fraction, and the major lipoprotein in plasma involved in cholesterol transport).
Purified apos (apoAI, apoAII
, apoB, apoCII, apoCIII, and apoE), anti-apoE polyclonal antibody (rabbit), anti-apoE monoclonal antibody, and horseradish peroxidase-conjugated anti-mouse IgG (goat) were purchased from Chemicon International Inc., Dako, Progen Biotechnik, and MBL Co., respectively.