Therefore, it was not possible to calculate the specific
antitryptic activity for RT, since obtaining this result depends on quantification of proteins.
The principle of serum alpha-1 AT assay is that the
antitryptic proteins of serum inhibit the hydrolysis of alpha-N-benzoyl-DL-arginine-P-nitroanilide (BAPNA) by trypsin in Tris buffer.
synthetic substrate for measuring the
antitryptic activity of soybean samples.
Measurement of
antitryptic activity: The
antitryptic activity of inhibitors was estimated by measuring the % inhibition activity of trypsin, using synthetic substrate benzoyl-DL-arginine-p-nitroanilide (Lin et al., 1991).
synthetic substrates for measuring
antitryptic activity of soya bean samples.
Quantification may be accomplished by a variety of methods, the most commonly used techniques being immunonephelometry and
antitryptic activity (14).
In the present study, we report for the first time
antitryptic activity in the seed extracts of Butea monosperma (Flame of Forest) and have partially characterized it for its thermal and pH stability in ammonium sulphate fractionated and dialyzed sample.
The results observed in this study, indicates that irradiation treatment has a substantial effect on the
antitryptic activity naturally present in soybeans.
Kakade ML, Simons N, Liener IE (1969) An evaluation of natural of synthetic subtracts for measuring the
antitryptic activity of soybean samples.
This protein has an
antitryptic activity that is acid and thermolabile.