The filter for the number of A/U in the 5'-end pentamer of the antisense strand (f-AU).
Two points are gained if the 5'-end antisense strand of a siRNA sequence starts with U.
This differential stability ensures that the antisense strand is incorporated into the RISC complex, reducing the unwanted off-target effect caused by the sense-strand [10,17,19,21,24,27,28,29].
To switch the roles of the two helix partners, researchers splice a promoter into the beginning of the sequence, then flop that piece of DNA so that the sense strand extends backward and the antisense strand
([dagger]) Exact mass measurements for the sense and antisense strands
of the dsDNA amplicon reported.
Researchers can order primers to investigate the SNP of interest on both the sense and antisense strands
as well as explore whether the template DNA is heterozygous or homozygous for a given allele.
In other cases, the bound antisense strands catalyze specific chemical reactions that actively degrade the targeted mRNA.
in Woodlands, Texas, have designed antisense strands of DNA that migrate directly into the cell's nucleus.
Researchers say they have just begun to assess the behavior and safety of so-called second-and third-generation oligos -- antisense strands chemically modified for enhanced stability or therapeutic potential in the body.
We tested both sense and antisense strands as hybridization probes.
Comparison of sense and antisense strands as hybridization probes.
When a sequence variation is present in the heterozygous state, the classical SSCP picture would be of four single-stranded bands, the sense and antisense strands
of the "normal" allele, and the altered mobility sense and antisense strands
of the "variant" allele.