antisense RNA


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an·ti·sense RNA

the transcription product of the DNA antisense strand; it can play a role in the inhibition of translation.
See also: antisense DNA.

antisense RNA

RNA molecules transcribed, not from DNA in the usual manner, but from DNA strands complementary to those that produce normal messenger RNA. Antisense RNA occurs in nature and is inhibitory on gene action. It can be produced synthetically and offers such therapeutic possibilities as turning off viral genes.

antisense RNA

single-stranded RNA which can hybridize to complementary bases in a target NUCLEIC ACID molecule and inhibit its function. Where the target is the RNA transcript of a gene (i.e. mRNA), copied from the SENSE STRAND of DNA, the antisense RNA is a counter-transcript, copied from the antisense strand (see ANTICODING STRAND of the DNA, and complementary to the mRNA. HYBRIDIZATION between the two transcripts can block the EXPRESSION of the GENE. Antisense RNA regulates various systems, such as the REPLICATION of certain PLASMIDS and the transfer of some plasmids by CONJUGATION. It has also been exploited in GENETIC ENGINEERING, to produce, for example, TRANSGENIC tomatoes, in which the antisense RNA inactivates expression of genes coding for ENZYMES that are involved in the softening and rotting of the fruit. In this way the shelf-life of the tomatoes has been extended. Another example of the use of antisense is in the development of variants of certain flowering plants, that show reduced or erratic coloration of the FLOWERS.
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HOTAIR (HOX transcript antisense RNA), which is 2.2 kb in length and is located on 12q13.13, is a long noncoding RNA formed by HOXC gene transcription.
(6) Nonstandard abbreviations: aRNA, antisense RNA; ssDNA, single-stranded DNA; dsDNA, double-stranded DNA; IVT, in vitro transcription; 2D-SDE, 2-dimensional strandness-dependent electrophoresis.
In one representation, the RNA synthesis from a DNA strand may hit the transcription of the other strand, suppressing the transcription of antisense RNA sense.
Adenovirus-mediated transfer of HPV 16 E6/E7 antisense RNA to human cervical cancer cells.
Some specific topics examined include clinical application of RNAs for cancer therapy, selection of target sites in RNA for antisense agents, and natural antisense RNA in mammals.
A species of small antisense RNA in postranscriptional gene silencing in plants.
The work that won Mello, 45, and Fire, 47, the coveted prize began in the mid-1990s when they and others were experimenting with "antisense RNA," a then-promising method for modulating the activity of genes inside cells.
The corresponding digoxigenin-labeled antisense RNA probes were prepared from pGEM-1 containing a BamH1-EcoR1 956-bp insert consisting of exon 3 and flanking intron sequences from the rat IGF-1 gene, and the pTRI-IGFR-human transcription template containing a 236-bp cDNA fragment of the human IGF-1R gene spanning exons 8-7 (Ambion, Austin, TX, USA).
One method, antisense RNA technology, proved to be unreliable.
A species of small antisense RNA in posttranscriptional gene silencing in plants.
This could possibly be explained by: (1) different localization of the endogenous OMT mRNA and the antisense OMT mRNA molecules within the cell preventing them from forming a duplex and triggering degradation, or (2) the detected antisense RNA molecules possibly being transcribed in different cell types.
Frechet foresees wide application for the technique in delivering antisense RNA, proteins or genes into cell interiors, and integrating with current methodologies.