antisense


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Related to antisense: Antisense strand, Antisense drugs

strand

 [strand]
a thread or fiber or a structure resembling one.
antisense strand the strand of a double-stranded nucleic acid that is complementary to the sense strand; in DNA it is the template strand on which the mRNA is synthesized.
sense strand the strand of a double-stranded nucleic acid that encodes the product; in DNA it is the strand that encodes the RNA, having thus the same base sequence except changing T for U in the RNA. See also antisense strand.

an·ti·sense DNA

the strand of DNA complementary to the one bearing the genetic message and from which it may be reconstructed; a DNA sequence complementary to a portion of mRNA; used as potential therapeutic agent to stop transcription or translation of pathogens or inappropriately expressed host gene.

antisense

(ăn′tē-sĕns′, ăn′tī-)
adj.
Of or relating to a nucleotide sequence that is complementary to a sequence of messenger RNA. When antisense DNA or RNA is added to a cell, it binds to a specific messenger RNA molecule and inactivates it.

gene therapy

Molecular medicine Treatment of disease by replacing, altering or supplementing the genetic structure of either germline–reproductive or somatic–nonreproductive cells a structure that is absent or abnormal and responsible for disease; any of a group of techniques in molecular biology, in which a gene of interest is manipulated, either by mutational inactivation–eg, the 'knock-out mouse', or by replacement, if it causes a particular disease; GT encompasses any therapy that specifically targets the core defect in inherited diseases, either by affecting somatic cells or germ line cells which are usually inserted into the host's genome; strategies for GT include
1. Introduction of a recombinant retrovirus with the missing gene, the promoter, and the gene regulator sequence in the 'package', and.
2. Implantation of the colonies of cells producing the missing factor(s)–eg, α1-antitrypsin deficiency with the missing enzyme introduced into 'carrier' fibroblasts
Gene therapy strategies
Antibody genes Interfere with cancer-related protein activity in tumor cells
Antisense Block synthesis of proteins encoded by a defective gene in the host
Chemoprotection Add proteins to cells that protect them from the toxic effect of chemotherapy
Immunotherapy Enhance host defense against cancer
Oncogene downregulation Turn off genes involved in uncontrolled growth and metastases of tumor cells
Suicide gene/pro-drug therapy Insert proteins that metabolize normal drugs and ↑ their toxicity to proliferating–ie tumor cells
Tumor suppressor genes Replace defective/deficient cancer-inhibiting genes
.

an·ti·sense

(an'tē-sens)
Pertaining to the strand of a double-stranded DNA or RNA molecule that is complementary to the sense strand.
References in periodicals archive ?
Spinraza is an antisense oligonucleotide (ASO), using Ionis Pharmaceutical Inc.'s proprietary antisense technology, that is designed to treat SMA caused by mutations or deletions in the SMN1 gene located in chromosome 5q that leads to SMN protein deficiency.
The specific antisense significantly decreased the overexpression of a substance called amyloid beta protein precursor, which normalized the amount of amyloid beta protein in the body.
For growth parameters and electrolyte leakage data, comparison was made between wild type and antisense transgenic plants under abiotic stress and under control conditions.
To address the functional role of LHR expression, we used an in vitro antisense strategy to decrease the level of LHR protein during oocyte maturation.
A 10-second pulse of ultraviolet light breaks the bond, liberating the antisense strip and allowing it to clamp down on the target gene product.
Delivery of a therapeutic dose of the antisense agent was achieved safely without any adverse systemic effects.
"This partnership with DTRA and the DoD serves as validation of AVI's proprietary NEUGENE antisense approach to gene silencing in general, as well as specific confirmation of AVI's concept of rapid response therapeutics," said Alan P.
Building on our 17 year history in oligonucleotide manufacture and analysis, Dalton can now use its expertise in synthesis and manufacturing of antisense oligonucleotides and their analogs to produce cGMP grade oligonucleotides as required for human clinical testing.
A nested reverse transcription-polymerase chain reaction (RT-PCR) was conducted to amplify part of open reading frame 2 (ORF2), which corresponds to nucleotides (nt) 5939-6297 of the genotype 1 HEV genome (GenBank D10330), with external sense primer HEV-F1 (5'-TAYCGHAAYCAAGGHTGGCG-3') and antisense primer HEV-R2 (5'-TGYTGGTTRTCRTARTCCTG-3').
The central nucleus of the inferior colliculus was bilaterally injected with replication-defective herpes virus (ICP4-; 2 x [10.sup.6] plaque forming units) encoding [GABA.sub.A] a-1 subunit in the antisense; lentivirus vectors (multiply attenuated, replication defective and self-inactivating) encoded [GABA.sub.A] a-1 sense or antisense (10% lacZ-encoding virus; controls 100% lacZ-encoding virus or vehicle), or GAD sense.
AVI BioPharma will test its NeuGene antisense drug, called AVI-4020, to treat West Nile patients suffering serious neurological impairment.
In this research, we wanted to determine whether antisense strategy would downregulate OMT activity, decrease lignin content, and increase forage digestibility to improve monocot forage quality.