In order to detect cytochrome c, lysing was performed on ice for 30 min in a buffer, consisting of HEPES (20 mM), NaCl (500 mM), MgCl2 (1.5 mM), 20 % glycerol, 0.1 % triton X100, EDTA (0.2 mM), aprotinin (1.7 mg/mL), antipain
(1.5 mM), pepstatin (1 mM), dithiothreitol (DTT, 1 mM), phenylmethylsulfonyl fluoride (PMSF, 0.5 mM), and 0.5 % sodium dodecyl sulfate (SDS).
PICs (e.g., aprotinin, leupeptin, antipain
, pepstatin A, phenylmethylsulfonyl fluoride, EDTA, and thimerosal) can avoid proteolysis through the inhibition of serine-, cysteine-, aspartic-, and metallo-proteases.
medications were the most commonly used medicine followed by respiratory drugs and gastrointestinal drugs.
NTS samples were homogenized in an ice-cold lysing buffer containing 20 mM Tris-HCl (pH 6.8), 150 mM NaCl, 10% glycerol, 1% NP-40, and 8 [micro]L/mL inhibitor cocktail (125 mM PMSF, 2.5 mg/mL aprotinin, 2.5 mg/ mL leupeptin, 2.5 mg/mL antipain
, and 2.5 mg/mL chymostatin).
Mitochondria and microsomes from freshly extracted mouse liver were prepared by differential centrifugation and suspended in 20 mM [K.sub.2]HP[O.sub.4] buffer containing 20% glycerol with added leupeptin, pepstatin, antipain
, and PMSF as described previously [29, 30].
Li et al., "Analgesic effect of electroacupuncture on complete Freund's adjuvant-induced inflammatory pain in mice: A model of antipain
treatment by acupuncture in mice," Japanese Journal of Physiology, vol.
We toured its packaging facilities as well as the production line of the boxed set of antipain
sprays, which use presumably famous Chinese sports figures (soccer players, as far as we could tell) as endorsers.
Cells were washed twice with phosphate-buffered saline and lysed in lysis buffer (10 mM Tris-HCl (pH 7.4), 1 mM EDTA, 1 mM EGTA, 5 [micro]g/ml aprotinin, 5 [micro]g/ml leupeptin, 5 [micro]g/ml pepstatin A, 5 [micro]g/ml antipain
, 50 mM NaF, 2 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 0.15 M NaCl, 1% Triton X-100, and 0.5 mM phenylmethylsulfonyl fluoride).
small pieces of wood for finger splints, Castoria, homemade cotton balls, Vicks Rub, Red lineament, Rawleighs or Watkins lotion, Antipain
oil, and a jar of goose grease and fever pills." According to writer Lori Scharfenberg, Eidse also sold "...
Cultured cells were collected and washed with PBS and suspended in extraction buffer (25 mM HEPES (pH 7.4), 50mMNaCl, 1% Triton X-100, 10% glycerol, 0.1mMPMSF, 0.1 mM pepstatin, 0.1 mM antipain
, 0.2 mM leupeptin, 10 [micro]g/mL aprotinin, 1mM benzamidine, 15mMNaF, and 1mM[Na.sub.3]V[O.sub.4]).
After stimulation, the cells were washed with ice-cold PBS, harvested, and resuspended in 300 [micro]L of hypotonic buffer A (10 mM HEPES, pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 1 mM PMSF, and 10 [micro]g/mL of leupeptin, antipain
, aprotinin, and pepstatin) for 10 min on ice.
8-bromoguanosine 3',5-icyclic monophosphate (8-Br-cGMP), Phenylmethylsulfonyl fluoride, orthovanadate, chymostatin, leuptin, antipain
, pepstatin A, trypan blue, Tween-20, mouse anti-[alpha]-tubulin, 2-Propanol, chloroform, and TRI Reagent were purchased from Sigma Chemical (St Louis, MO, USA).