antigen retrieval

antigen retrieval

A process in which tissues are heated with a microwave oven, steam (pressure) cooker, autoclave or ultrasound, which causes antigens that are difficult or impossible to stain in formalin-fixed paraffin sections of tissue to become readily stainable by standard immunohistochemical methods.
References in periodicals archive ?
Prepare sections between 3-4micrometer thickness on Poly-L-Lysine coated slides or silanized slides to keep the samples from detaching, especially during antigen retrieval approaches.
The effect of type of incubation and two different antigen retrieval methods on the immunodetection of Mannheimia hemolytica antigens in archived caprine lung tissues.
Second, antigen retrieval was done by heating the sections to 95[degrees]C in 0.01 mol/L citric acid buffer (pH 6.0) for 15 min.
Tenders are invited for Procurement Of Antigen Retrieval Machine FOR THE YEAR 2018-19
Slides were treated in PT-Link machine (Dako, Glostrup, Denmark), which includes deparaffinization, rehydration, and antigen retrieval for 1 h and then cooled for 15 min.
Standardization in immunohistochemistry: the role of antigen retrieval in molecular morphology.
These steps were followed by heating the sections in a microwave oven for antigen retrieval using citrate buffer (pH 5.5) as previously described (Tacha and Chen, 1994).
Sections of resected specimens were cut with 3-5 um thickness and transferred to antigen retrieval solution (Tris-EDTA Ph 9).
Under-slide heating also makes it difficult to determine the antigen retrieval (AR) solution temperature at the tissue level, limits the volume of AR solution (which leads to evaporation issues), and makes the heaters vulnerable to corrosion and failure due to the hostile under-slide environment.
Antigen retrieval was performed by pressurized heating at 125[degrees]C in a citrate-buffered solution at pH 6.0 (Dako North America Inc., Carpinteria, CA) for Ki-67 and p63, or in an ethylenediamine tetra-acetic acid buffer solution at pH 9.0 for the estrogen receptor (ER) and progesterone receptor (PR) (Dako North America Inc., Carpinteria, CA, USA).
Antigen retrieval was performed by immersing the slides in citrate buffer (pH 6.0; 0.01 M) or EDTA (pH 8.0; 0.01 M) and subjecting them to 97[degrees]C in a water bath for 30 minutes.