Camasses et al., "The cell proliferation antigen Ki-67
organises heterochromatin," eLife, vol.
Wu, "Correlation between the uptake of [sup.18]F-fluorodeoxyglucose ([sup.18]F-FDG) and the expression of proliferation-associated antigen Ki-67
in cancer patients: a meta-analysis," PLoS One, vol.
Kljucne rijeci: Glioblastom--terapija; Antikancerogeni lijekovi; Ljudi; Selen; Selenometionin; Stanicna proliferacija--ucinci lijekova; Stanicna smrt--ucinci lijekova; antigen Ki-67
The primary antibody against the antigen Ki-67
was diluted 1: 200 in UltrAb Diluent (Thermo Scientific, Fremont, USA).
Immunohistochemical assessment of the nuclear antigen Ki-67
can be useful in the differential diagnosis between adrenocortical adenoma and carcinoma.
Expression of Ki-67 and CD31 was analysed in formalin fixed, paraffin-embedded sections with the method of indirect immunoperoxidase with the use of polyclonal rabbit antibodies to human antigen Ki-67
and CD31 ("Dako", Denmark).
Immunohistochemical evaluation of cell proliferation antigen Ki-67
and apoptosis-related proteins Bcl-2 and caspase-3 in oral granular cell tumor.
Expresion of estramustinebinding protein (EMBP) and the proliferation associated antigen Ki-67
in prostatic carcinomas.
The antibodies were used according to machine vendor's protocol; for the native nuclear proliferation antigen Ki-67
, the antibody MIB-1 (dilution 1: 200, Immunotech, Marseille Cedex, France) was used, and for p53, the antibody DO-7 was used (anti-human p53 protein, dilution 1:300, Dako).
Proliferation is widely estimated by the immunohistochemical assessment of the nuclear antigen Ki-67
. Apoptotic cells are frequently quantified by counting morphologically positive cells on an H&E stain or evaluating the number of TUNEL-positive cells per 1000-2000 cells.
Prognostic value of the proliferation-related antigen Ki-67