In the
antibiotic sensitivity test, the isolate was found to be resistant to ampicillin, amoxycillin-clavunate, cefazolin, cefoxitin and trimetorprim-sulphametoxasol and sensitive to amikacin, aztreonam, cefuraxim, ceftriaxon, ceftazidim, cefepime, ciprofloxacin, imipenem, meropenem and piperacillin-tazobactam.
With increase in resistance for common antibiotic used in the field condition, the potential of alternative antibiotics found effective in in vitro
antibiotic sensitivity test may be considered for treatment against mastitis.
Antibiotic sensitivity tests were conducted to evaluate the sensitivity of causative organisms for antibiotics and presented in Table 3 (Fig.
Further
Antibiotic sensitivity test and antimicrobial activity of the organism was done.
Bacteriological studies and
Antibiotic sensitivity testThe
antibiotic sensitivity test revealed bacterial sensitivity to Ceftriaxone (++++), Oxytetracycline (+ + +), Enrofloxacin (+ +), Gentamicin (++), Ampicillin (+) and Ciprofloxacin (+) after 48 hours of incubation.
Vaginal cytology was conducted using Leishman staining and further samples were processed for
antibiotic sensitivity test. Swab was placed in nutrient broth and incubated.
Once the causative organism was isolated, it's culture on Mueller-Hinton agar was subjected to
antibiotic sensitivity test using standard antibiotic discs.
All isolates identified were subjected to
antibiotic sensitivity test (AST) by disc diffusion method to different antibiotics (Bauer et al., 1966).
There is an urgent need to enhance awareness among the dairy farmers regarding importance of
antibiotic sensitivity test in choosing the appropriate drug for treating mastitis to avoid emergence of multi drug resistant strains and improve public health.
Findings of the present study emphasize the need of
antibiotic sensitivity test for determining most suitable antimicrobial agent including for newly introduced antibiotics for therapy of mastitis.
The antibiotics were then continued as per
antibiotic sensitivity test.