In the antibiotic sensitivity test
, the isolate was found to be resistant to ampicillin, amoxycillin-clavunate, cefazolin, cefoxitin and trimetorprim-sulphametoxasol and sensitive to amikacin, aztreonam, cefuraxim, ceftriaxon, ceftazidim, cefepime, ciprofloxacin, imipenem, meropenem and piperacillin-tazobactam.
With increase in resistance for common antibiotic used in the field condition, the potential of alternative antibiotics found effective in in vitro antibiotic sensitivity test
may be considered for treatment against mastitis.
Antibiotic sensitivity tests
were conducted to evaluate the sensitivity of causative organisms for antibiotics and presented in Table 3 (Fig.
Further Antibiotic sensitivity test
and antimicrobial activity of the organism was done.
Bacteriological studies and Antibiotic sensitivity test
The antibiotic sensitivity test
revealed bacterial sensitivity to Ceftriaxone (++++), Oxytetracycline (+ + +), Enrofloxacin (+ +), Gentamicin (++), Ampicillin (+) and Ciprofloxacin (+) after 48 hours of incubation.
Vaginal cytology was conducted using Leishman staining and further samples were processed for antibiotic sensitivity test
. Swab was placed in nutrient broth and incubated.
Once the causative organism was isolated, it's culture on Mueller-Hinton agar was subjected to antibiotic sensitivity test
using standard antibiotic discs.
All isolates identified were subjected to antibiotic sensitivity test
(AST) by disc diffusion method to different antibiotics (Bauer et al., 1966).
There is an urgent need to enhance awareness among the dairy farmers regarding importance of antibiotic sensitivity test
in choosing the appropriate drug for treating mastitis to avoid emergence of multi drug resistant strains and improve public health.
Findings of the present study emphasize the need of antibiotic sensitivity test
for determining most suitable antimicrobial agent including for newly introduced antibiotics for therapy of mastitis.
The antibiotics were then continued as per antibiotic sensitivity test