Occurrence of OBI in total anti-HBc
positive subjects varies in different parts of India [0% in Chandigarh, 20.87% in New Delhi (Northern India), 30% in Ganjam and 21% in Kolkata].
Clinical significance of 'anti-HBc
alone' in human immunodeficiency virus-positive patients.
A amostra soropositiva para o HBsAg era anti-HBc
IgM negativa, HBeAg negativa e anti-HBeAg positiva.
The samples were tested for serological markers of HBV infection (HBsAg, anti-HBc
and anti-HBs) using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructors (Acon, San Diego, CA, USA).
When the serum was positive for either HBsAg or anti-HBc
, HBV DNA levels were analyzed using real-time PCR.
In two other investigations conducted in the State of Sao Paulo, OBI was diagnosed by nested PCR in sera of 12 of 50 (24%) (20) and 15 of 106 (14%) anti-HBc
positive patients with chronic HCV infection (21).
It is also suggested in the literature that all patients receiving chemo- and immunotherapy should be tested at least once for anti-HBc
antibodies before starting therapy and monitored periodically for ALT elevations22.
The diagnosis of OBI was made when either real-time or nested PCR showed HBV DNA in the absence of detectable HBsAg, regardless of being seropositive or negative either for anti-HBc
or anti-HBs or both markers.
Among the 2195 collected blood samples, 268 were anti-HBc
IgM was present in 7 out of 96 subjects who tested positive for anti-HBc