Murine mAb against anthrax lethal factor (LF8) was developed and purified in our lab, as described previously .
Crown et al., "Novel chimpanzee/ human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody," Infection and Immunity, vol.
Cao, "Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model," Human Antibodies, vol.
Duesbery, "Biological and biochemical characterization of anthrax lethal factor, a proteolytic inhibitor of MEK signaling pathways," Methods in Enzymology, vol.
Bound to these molecules is a protein fragment, or peptide, that gets cleaved in two in the presence of anthrax lethal factor, a toxin produced by the bacterium.
After screening 10,000 chemicals in 3 days, the researchers found one compound, called DS-998, that blocked the activity of anthrax lethal factor. In a subsequent test, the compound protected lab-grown human cells that later were exposed to the toxin.