FITC annexin V
and propidium iodide (PI) staining were performed using the FITC annexin V
Apoptosis Detection Kit from BD Biosciences (USA) to determine the level of apoptosis.
Apoptosis and necrosis in the circumventricular organs after experimental subarachnoid hemorrhage as detected with annexin V
and caspase 3 immunostaining.
, a potent anticoagulant that displaces phospholipid-dependent coagulation factors, is located on placental trophoblast cells and vascular endothelium.
(A5) has been widely used in basic and clinical research as an apoptosis marker in conjunction with propidium iodide to distinguish between apoptotic and necrotic cells.
However, to be accurate, it is "cell death imaging" and not "apoptosis imaging." The typical compound of PS targeted carriers is annexin V
, a 36kDa human protein with a nanomolar affinity for membrane-bound PS [3-5].
, propidium iodide and Hoechst staining: For Annexin V
, staining suspensions of cumulus cells from individual oocytes were incubated with 1 [micro]l of Annexin V
(2.5 [micro]g/ml) for 15 min at 37 [degrees]C in the dark.
 demonstrated that the flow cytometric assessment of necrotic and early apoptotic cells by staining against 7-aminoactinomycin (7-AAD) and Annexin V
(AnnV), respectively, is a feasible method for predicting CFU results.
For apoptosis, plot quadrants were set using unstained cells for every sample such that the negative annexin V
cells and 7-AAD negative population lay in the first decade of the Y and X axis.
Additionally, the increased levels of four urinary PAH metabolites were significantly associated with decreased vital Annexin V
negative sperm counts.
was shown to be useful for detection of apoptotic cells because of its binding affinity for negatively charged phospholipids like phosphatidylserine, exposed at the beginning of apoptotic process [13-15].
Another marker for apoptosis, annexin V
, was used to evaluate membrane changes with fluorescence microscopy and flow cytometry.