The spindle cells contained moderate anisokaryosis
with rounded to fusiform, often vesicular, nuclei; poorly defined cytoplasmic borders; and a low mitotic rate.
There is an increase in the number of mitoses, and dyskaryosis, anisokaryosis
, and dyschromatosis are present (figure 3).
The characteristic cytologic features of conventional melanoma are: high cell-yield, predominant epithelioid/plasmacytoid cells with eccentrically placed nuclei and a dissociated pattern, abundant cytoplasm, cytoplasmic melanin pigments, prominent anisokaryosis
, macronucleoli, intra-nuclear cytoplasmic inclusions, and variable numbers of bi- and multinucleated cells.
Neoplastic cells were pleomorphic with marked anisocytosis and anisokaryosis
Hurthle cell atypia characterized by nuclear anisokaryosis
and varying degrees of nuclear pleomorphism has been well described in Hashimoto's thyroiditis.
Dysplastic squamous and columnar epithelial cells showed pleomorphism with anisokaryosis
, enlarged and coarsely structured nuclei, and prominent nucleoli (Figure 1).
The round to slightly elongate neoplastic cells had varying nuclear to cytoplasmic ratios with moderate anisocytosis and anisokaryosis
Anisocytosis and anisokaryosis
were moderate, while pleomorphism and anaplasia were low-grade.
Variable moderate anisokaryosis
and anisocytosis were observed, with a mitotic index of 33 per 10 x 400 fields.
There was moderate anisocytosis, anisokaryosis
, and 5 mitotic figures per 10 X400-power fields.
The microscopic changes observed in all 30 birds were hepatocellular anisocytosis (ND in 26 birds, 0 in 1 bird, 1+ in 3 birds) and anisokaryosis
(ND in 1 bird, 0 in 4 birds, 1+ in 18 birds, 2+ in 6 birds, 3+ in 1 bird), hepatic lipidosis (0 in 24 birds, 1+ in 4 birds, 2+ in 1 bird, 4+ in 1 bird), hepatocellular necrosis (0 in 26 birds, 1+ scattered necrosis in 1 bird, and focal necrosis in 3 birds), heterophilic hepatitis with variable degrees of lymphocytic or histiocytic infiltrates (0 in 6 birds, 1+ in 20 birds, 2+ in 4 birds), glycogen-laden hepatocytes (0 in 3 birds, 1+ in 14 birds, 2+ in 4 birds, 3+ in 7 birds, 4+ in 2 birds), and iron-laden hepatocytes (0 in 13 birds, 1+ in 7 birds, 2+ in 8 birds, 3+ in 2 birds).
The cells had indistinct cell margins and abundant, finely granular, pale eosinophilic cytoplasm with polygonal nuclei that contained reticular chromatin and prominent, often multiple nucleoli, and marked anisokaryosis