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The pI-17[alpha] expression levels correlated positively with the frequency of anaphase aberrations (p = 0.005): the higher the levels of the centromeric noncoding RNAs, the higher the percentage of cells that showed bridges and/or lagging chromosomes.
However, approximately 20% of the anaphase cells analyzed after transfection with each chromosome a vector contained bridging or lagging chromosomes in HT1080 and HUES-10 cells (Figures 1(b) and 1(c)).
Laggard chromosomes (Figure 1D and J) were visualized in MPP I and MPP II plants, affecting 16.83% of meiocytes in anaphase I and 35.90% in anaphase II, respectively.
Chromosome grouping during metaphase I and anaphase I led to the formation of chromosome stickiness bridges in telophase I (Figure 1G), observed only in MPP I.
The cells were recorded as normal or aberrant in the different stages of the cell cycle namely: interphase, prophase, metaphase, anaphase or telophase.
Metaphases II showed chromosome numbers that confirmed the reductional segregation of the chromosomes during the preceding anaphase I, including the asynaptic X chromosomes present in the cells with 30 = 14II + 2X.
With respect to the results listed in Table 2, the orange and grape juices of the five food companies, at both exposure times considered, induced significant formation of mitotic spindle changes, represented in this study by colchicine metaphase and anaphase and telophase bridges, proving to be genotoxic, and chromosome breaks, characterized by the formation of micronuclei.