Sixty min after treatment, they were evaluated for mechanical hyperalgesia by digital analgesimeter (digital von Frey) and muscle strength (grip strength meter) for 7 consecutive days.
Additionally, we also evaluated the analgesic effect of the chronic treatment with CT-[beta]CD in animals induced with non inflammatory chronic muscle pain, which is suggested to be an animal model of FM, for 7 days through the digital analgesimeter, once, in this model, there is a significant decrease in mechanical withdrawal threshold of the paw, being interpreted as secondary hyperalgesia (Da Silva et al., 2010; Sluka et al., 2001).
As described in Figure 3, the animals were subjected to the analgesimeter
(Anal 1) and formalin (FT1) tests before the beginning of substance treatment to evaluate the basal response of each one to the different experimental procedures.
We measured mechanical hyperalgesia using a Randall-Selitto analgesimeter
for mouse (Ugo Basile, Varese, Italy).
The pain threshold in the rat was determined with an analgesimeter
(Ugo Basile, Varese, Italy) as described .
(1971), using an Ugo-Basile analgesimeter
. Agar 1% was administered to the control group, acetylsalicylic acid (100 mg/kg body wt.) was used as reference drug.
The test consisted of ascertaining the ability of the rat to support pressure applied to the posterior paw using a Ugo Basile analgesimeter
. Sixty, 120 and 180 minutes after treatment, the analgesia coefficient was calculated using the following formula (Oga et al., 1984):