amplimer

OLIGONUCLEOTIDE used as a PRIMER of DNA synthesis in the POLYMERASE CHAIN REACTION.

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An amplimer of 232 bp for AHCYTOEN gene was obtained from both field and reference samples.

Detection of Pathogenic Aeromonas hydrophila from Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey

The primer sets were selected to amplify approximately 500 base pair (bp) amplimer in the COI gene.

A preliminary report on DNA barcoding and phylogenetic relationships of certain public health important mosquito species recorded in rural areas of south India

Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates.

Characterization of gyrA and gyrB mutations and fluoroquinolone resistance in Mycobacterium tuberculosis clinical isolates from Hubei Province, China

A 535bp amplimer was generated with primers MTLa1-F (5'-TGAAAATGAAGACAATGCGA-3') and MTLa1-R (5'CATCTTTTTCTGCTATCAATTC-3') in the presence of MTL type a DNA, and a 615-bp product resulted from primers MTL[alpha]1-F (5'-ATGAATTCACATCTGGAGGC-3') and MTL[alpha]1-R (5'-CTGTTAATAGCAAAGCAGCC-3') in the presence of MTL type [alpha] DNA.

Genetic differences between avian and human isolates of Candida dubliniensis

The second dystrophin amplimer group consists of 38 amplicons of symmetric exons [(0,0), (1,1), (2,2)].

Log-PCR: a new tool for immediate and cost-effective diagnosis of up to 85% of Ddstrophin gene mutations

The primer set Aca16Sf1010 (5'-TTATATTGACTTGTACAGGTGCT-3') and Aca16Sr1180 (5'-CATAATGATTTGACTTCTTCTCCT-3'), which was designed to give an amplimer of 161 bp, was used for detecting Acanthamoeba DNA (3).

Acanthamoeba encephalitis in patient with systemic lupus, India

We had difficulty resolving the 99-bp amplimer cut and uncut fragments from the 22 and 23mer PCR primers on 3% agarose, and the 176-bp product was more easily resolved, as was the 118-bp digestion product.

Accuracy and biological variation of human serum paraoxonase I activity and polymorphism (Q192R) by kinetic enzyme assay

An important result was that, as expected, Inv22 Southern blot patterns (distal and proximal) yielded the same Inv22-specific amplimer (559 bp) without significant differences in relative signal intensities.

Genotyping the hemophilia inversion hotspot by use of inverse PCR

5 [micro]M each amplimer, 100[micro]M deoxynucleotide triphosphates, 10 mM Tris-HCl (pH 8.

Characterization of the bell polymorphism in the glucocorticoid receptor gene

This is achieved by covalently attaching one primer to the microwell before amplification, thereby allowing colorimetric detection of a solid-bound amplimer carrying a detector tag after washes.

Solid-phase amplification for detection of C282Y and H63D hemochromatosis (HFE) gene mutations

The DHPLC analysts were blind to the nature and number of heterozygotes present in the sample, although a wild-type control for each amplimer was known.

Optimal temperature selection for mutation detection by denaturing HPLC and comparison to single-stranded conformation polymorphism and heteroduplex analysis

Here we present the integrated system Three-STAR, the amalgam of three techniques using a STAR annealing function amplimer.

A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping