amplification

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amplification

 [am″plĭ-fĭ-ka´shun]
the process of making larger, such as the increase of an auditory or visual stimulus, as a means of improving its perception.
DNA amplification artificial increase in the number of copies of a particular DNA fragment into millions of copies through replication of the segment into which it has been cloned, a type of nucleic acid amplification.
gene amplification a process by which the number of copies of a gene is increased in certain cells because extra copies of DNA are made in response to certain signals of cell development or of stress from the environment. In humans this process is seen most often in malignant cells.
nucleic acid amplification amplification of a specific nucleic acid sequence, such as to test for presence of a given virus or bacteria in a sample. Types include DNA amplification, ligase chain reaction, and polymerase chain reaction.

am·pli·fi·ca·tion

(am'pli-fi-kā'shŭn),
The process of making larger, as in increasing an acoustic or visual stimulus to enhance its perception.
[L. amplificatio, an enlarging]

amplification

(ăm′plə-fĭ-kā′shən)
n.
1. The act or result of amplifying, enlarging, or extending.
2. Physics
a. The process of increasing the magnitude of a variable quantity, especially the magnitude of voltage, power, or current, without altering any other quality.
b. The result of such a process.
3. Genetics The process by which extra copies of a gene or a DNA sequence are formed.

amplification

An in vivo (as in the fragile X syndrome) or in vitro (as in cloning or PCR) increase in the number of copies of a specific gene or DNA sequence of interest.

amplification

Molecular biology An in vivo–as in the fragile X syndrome or in vitro–as in cloning or PCR ↑ in the number of copies of a specific gene or DNA sequence of interest. See Cloning, DNA amplification, Exon amplification, Extreme amplification, Gene amplification, Linked linear amplification, PCR, PCR amplification of specific alleles, Solid phase amplification.

am·pli·fi·ca·tion

(am'pli-fi-kā'shŭn)
1. The process of making larger, as in increasing an auditory or visual stimulus to enhance its perception.
2. molecular biology Process of increasing the number of nucleic acid copies in a sample to millions within a short period.
[L. amplificatio, an enlarging]

amplification

The production of extra copies of a DNA sequence. These may be within the chromosomal sequence or outside it.

Amplification

A process by which something is made larger. In clotting, only a very few chemicals are released by the initial injury; they result in a cascade of chemical reactions which produces increasingly larger quantities of different chemicals, resulting in an appropriately-sized, strong fibrin clot.
Mentioned in: Hemophilia
References in periodicals archive ?
Centromeric paint and specific locus probes were used when needed to investigate MYCC and MYCN amplification and chromosome 9 centromere.
For all patients the RFA amplification was >100 000-fold, and the mean CV for the all analyses was 11.8%.
We further investigated the reproducibility of this finding in 16 MRSA strains isolated four years later in 2013, and found that the 3-, 4- and 5-primer MLVA amplification clustered the tested strains in a similar manner (Figure 2).
In recent years, there has been a growing interest in isothermal amplification methods (22) such as recombinase polymerase amplification (RPA) (23) and LAMP (21) for POC diagnostics.
Detection of Fibroblast Growth Factor Receptor 1 Amplification by Fluorescencein situhybridization
(8) Similarly, virtually all MPBC cases are negative for HER2 overexpression and amplification by either immunohistochemistry (IHC) and/or fluorescence in situ hybridization.
Indentification of such amplifications of the MET gene can be used for predicting therapeutic response, overall prognosis and cancer recurrence, and for ongoing monitoring.
Also PCR amplification of DNA directly from whole, undissected larvae and adults of the fruit fly Drosophila melanogaster was reported (Grevelding et al.
Thirty PCR cycles were run using ASTEC OC707 (ASTEC, Tokyo, Japan): denaturation step at 95[degrees]C for 1 min, annealing at 58[degrees]C for 1 min, and extension at 70[degrees]C for 2 min for amplification of the ttha1806 gene; and denaturation step at 95[degrees]C for 1 min, annealing at 50[degrees]C for 1 min, and extension at 70[degrees]C for 2 min for amplification of the ttha0122, ttha1300, and ttha1548 genes.
Its meaning can be roughly paraphrased as "you are already thinking about a referent and you are about to hear something more about it." It signals that what follows is a clarification, expansion, or amplification of a referent already introduced.
Among the techniques used to detect gene amplification are fluorescent in situ hybridization (FISH), array-based comparative genomic hybridization (array-CGH), and real-time PCR (qPCR).
Melanomas on sun-exposed skin rarely show such amplifications, and when present, tend to occur late in the malignant transformation of melanoma.