LineaRx believes linear DNA is ideal for diagnostic applications in that modifications to the target amplicon
made through the PCR process are faster, more efficient and less costly than traditional chemical derivatization.
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We retrospectively performed 16S amplicon
sequencing with MinlON.
RT-PCR amplification, genomic amplification, and amplicon
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This would contain on the order of 10 and 12 template copies (amplicons
); in other words, if a thorough cleaning reduced this by a million fold, you'd still have a million amplicon
copies "floating around," each of which could contaminate a reaction.
Data from other NGS assays (TruSeq Amplicon
Cancer Panel; Illumina) were also available for a subset of cases, (28) and were used for comparing results from overlapping regions.
There were noticeable variations in the I-PCR amplicon
patterns among the six brain compartments, and within each compartment, the MLV-ERV landscape patterns were not shared by the two mice examined.
The RT-PCR primers are described in Table 1 and the derived amplicon
encompasses nucleotides 2,071~ 3,370 of HIV-1 pol gene as referring to HIV-1 HXB-2 sequence (GenBank Accession number: K03455) [14, 15].
HRMA measures the decrease of fluorescence as double-stranded DNA denatures into single-stranded DNA, producing sequencespecific melting profiles that are dependent on amplicon
lengths and GC content (18-20).
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