The NEXTflex[TM] Amplicon
Panels allow researchers to sequence up to 2,000 amplicons
using a simple workflow with 100% coverage of targeted regions.
Contract notice: Tender for the supply of automated preparation of samples for single cell Genomics and Multiplex Library Preparation for Targeted Amplicon
Resequencing to the University of Birmingham.
Sequencing was performed using an Illumina TruSeq Custom Amplicon
size 250bp) on a MiSeq Personal sequencer with the MiSeq Control and Reporter softwares generating base calls, assembly and alignment of reads to the human reference genome (hg19), and variant calls, 91% (61 of 67) of which had a quality score of >/=Q30.
A cloned and sequenced 731-nt amplicon
(GenBank accession no.
In essence, amplicon
assays tend to be quick and affordable for small panels, while the longer baits of hybridization assays can be designed to provide greater accuracy for larger panels, as will be explained.
c] of an amplicon
usually lies approximately 1[degrees]C below the melting temperature ([T.
For instance, if there is a repeat region immediately adjacent to an exon, we would analyze the forward sequence from the amplicon
that excludes the repeat and the reverse reaction containing the repeat to obtain adequate coverage.
The primer part of the amplicon
is provided from outside.
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were visualized on the gel using the Promega (Madison, WI) silver-staining protocol (following manufacturer's instructions) and a 200 base pair (bp) DNA ladder as a marker to estimate amplicon
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The nucleotide sequences of the amplicon
obtained with a set of primers for the 4b core protein of fowl poxvirus revealed that the mynah poxvirus was phylogenetically related to wood pigeon poxvirus.