ampholytes


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Related to ampholytes: isoelectric point, zwitterion

ampholytes

see amphoteric sanitizing agents.
References in periodicals archive ?
Each serum sample (500 [micro]g of protein) was solubilized in 8 mol/L urea, 40 mL/L CHAPS, 40 mmol/L Tris, 2 mL/L Bio-Lyte 3/10 ampholytes (Sequential Extraction Buffer 2; Bio-Rad) with 5 mmol/L tris(2-carboxyethyl)phosphine reducing agent and incubated for 1 h at room temperature.
variability in gel conductivity, quality of carrier ampholytes, and electroendosmosis) make IEF inherently difficult to standardize.
In addition, sequestering of iron by the ampholytes during IEF might also occur.
5% ampholytes, pI range 3-7, at constant power (15 W) for 1 h at 4 [degrees]C (refrigerated circulating bath).
In reality, the pH gradient created by ampholytes is subject to disturbances by sample constituents that lead to deflections of the lines of equal pH (iso-pH) and produce bands that have been characterized as "frowns" or "smiles".
Urea, Ready-Mix IEF acrylamide/bisacrylamide (29:1 by weight), ammonium persulfate, and N,N,N,N-tetramethylethylenediamine were purchased from Amersham Biosciences, and the ampholytes Servalyt 2-4, 4-6, and 6-8 were purchased from Serva.
Serum containing ~5 [micro]g of total AFP was applied to a mixture of 50 g/L sorbitol, 100 mL/L glycerol, and ampholytes (6.
For example, isoelectric focusing (IEF) in immobilized pH gradients is compared with IEF in carrier ampholytes pH gradient.
Flatbed gels of 5% polyacrylamide containing ampholytes (pH range 4.
For oligonucleotide separation, His showed the best resolution compared with lysine or ampholytes, even if its buffering power was lower.