The present study aims to determine the morphological features of amphistome adults and eggs combined with examining pathological, hematological and bile biochemical response in buffaloes to Gigantocotyle explanatum infection.
The results demonstrate that morphological markers, pathological, hematological and bile biochemical studies are effective tools that could be used as complementary in diagnosis of amphistome infections in ruminants.
Gigantocotyle explanatum is one of neglected amphistome parasite infecting the bile duct of water buffalo (Ichikawa et al.
Although diagnosis of amphistome infection relies on detection of parasite eggs during faecal examination, it is impossible to identify which species is present from morphological analysis of the eggs alone.
Amphistome specimens providing the largest worm variability in their size, maturity and gravid uteri were used for characterization.