Detection of pyrogens adsorbed to intraocular lenses: Evaluation of limulus amoebocyte
lysate and in vitro pyrogen tests.
 Nonstandard abbreviations: BD, Becton Dickinson; TNF-[alpha], tumor necrosis factor [alpha]; LPS, lipopolysaccharide; RBC, red blood cells; PBMC, peripheral blood mononuclear cells; MAPK, mitogen-activated protein kinase; IL, interleukin; MAP, multiple analyte panel; CCL, chemokine ligand; MCP, monocyte chemotactic protein; PTX3, pentraxin 3; bDNA, branched DNA; LAL, limulus amoebocyte
Clotting cells and Limulus amoebocyte
lysate: an amazing analytical tool.
Evidence of the action of the amoebocytes
and the participation of different lectins in binding the larvae of the trematode with the amoebocytes
(Martins-Souza et al., 2006) can be tested by the technique of cultures of the amoebocyte
producing organ (APO) as first described by Barbosa et al.
Endotoxin was determined by limulus amoebocyte
lysate assay (QCL-1000, Cambrex).
Endotoxin analysis is usually carried out using the Limulus Amoebocyte
Lysate (LAL) assay.
Purity was assessed by endotoxin content (determined by chromogenic endotoxin assay, using a modified limulus amoebocyte
lysate and a synthetic color-producing substrate), which was considered acceptable in concentrations <0.1 IU/mL.
In a successful second career as head of Associates of Cape Cod, Watson and company produced that extract--limulus amoebocyte
lysate (LAL)--which is used by hospitals and medical device producers worldwide to detect bacterial contamination and ensure safe products.
Chromogenic Limulus amoebocyte
lysate assay for rapid detection of Gram negative bacteriuria.
MAFF scientists have published the protocols for four fully-validated tests: detection of irradiated herbs and spices using thermoluminescense technology; detection of irradiated bone-containing meat using electron spin resonance spectroscopy; detection of irradiated food containing fat by using 2-alkylcyclobutanone markers; and detection of irradiated poultry meat using the Limulus Amoebocyte
Lysate test in conjunction with a gram-negative bacterial count.
Endotoxin was measured using the limulus amoebocyte