3] Nonstandard abbreviations: BD, Becton Dickinson; TNF-[alpha], tumor necrosis factor [alpha]; LPS, lipopolysaccharide; RBC, red blood cells; PBMC, peripheral blood mononuclear cells; MAPK, mitogen-activated protein kinase; IL, interleukin; MAP, multiple analyte panel; CCL, chemokine ligand; MCP, monocyte chemotactic protein; PTX3, pentraxin 3; bDNA, branched DNA; LAL, limulus amoebocyte
Clotting cells and Limulus amoebocyte
lysate: an amazing analytical tool.
1986), who saw considerable mitotic activity in the Amoebocyte
Producing Organ (APO) during the first 48 hours after infection of B.
lysate test: an aid to the diagnosis in the septic neonate?
MAFF scientists have published the protocols for four fully-validated tests: detection of irradiated herbs and spices using thermoluminescense technology; detection of irradiated bone-containing meat using electron spin resonance spectroscopy; detection of irradiated food containing fat by using 2-alkylcyclobutanone markers; and detection of irradiated poultry meat using the Limulus Amoebocyte
Lysate test in conjunction with a gram-negative bacterial count.
Endotoxin was measured using the limulus amoebocyte
Tenders are invited for Limulus Amoebocyte
Lysate (10 Test Vial)
To measure airborne endotoxin concentrations, endotoxin assays were performed on the cotton dust sample filters using the Limulus amoebocyte
lysate assay, chromogenic method (Kinetic-QCL; BioWhittaker, Walkersville, MD, USA) (Olenchock et al.
All P-Dex preparations were tested for endotoxin contamination using the Limulus amoebocyte
lysate assay (Seikagaku, Tokyo, Japan).
It is a waste product produced during the preparation of Limulus amoebocyte
lysate (LAL), a clotting agent used worldwide to test for bacterial contamination of pharmaceuticals and implantable medical devices.
All samples were first subjected to assays for endotoxin contamination (Limulus amoebocyte
lysate method), which may stimulate immune cells, of which we found no evidence.