Crystal structure of a dodecameric tetrahedral-shaped aminopeptidase
We analyzed mRNA expression levels of Filamin 3 and Aminopeptidase
N by RT-qPCR as previously reported (17).
2D = grown on plastic, 2.5D = grown on transwell inserts, 3D = grown in matrigel sandwich, OAT = organic anion transporter, MRP = multidrug resistance protein, OCT = organic cation transporter, CTR1 = copper transporter 1, MATE = multidrug and toxin extrusion protein, OCTN = organic cation/ carnitine transporter, PGP = multidrug resistance protein/[gamma]-glycoprotein, BCRP = breast cancer resistance protein, SGLT = sodium/glucose co-transporter, GLUT1 = glucose transporter 1, CUBN = cubilin, AQP1 = aquaporin 1, GGT1 = y-glutamyltransferase 1, ALPL = alkaline phosphatase, LAP3 = leucine aminopeptidase
3, BF = bright field, RFU = relative fluorescence unit.
Matsumoto et al., "Characterization of recombinant human adipocyte-derived leucine aminopeptidase
expressed in Chinese hamster ovary cells," Journal of Biochemistry, vol.
Keller, "Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase
IRAP," American Journal of Physiology-Endocrinology and Metabolism, vol.
Trichoplusia ni resistance to Cry1Ac toxin in this strain was related to aminopeptidase
N (APN1 and APN6) genes by a trans-regulatory mechanism, where APN1 was down-regulated and APN6 was up-regulated (Zhang et al.
Expression of ENPEP, the gene which encodes aminopeptidase
A, a major Ang II metabolizing enzyme, was expressed at a moderate level in normal lung tissue with a statistically significant (p < 0.001, corrected for multiple comparisons, Table 2) 32% reduction in its expression in lung tumor tissue (Figure S1).
Jardinaud et al., "First discrete autoradiographic distribution of aminopeptidase
N in various structures of rat brain and spinal cord using the selective iodinated inhibitor [125I]RB 129," Neuroscience, vol.
Additionally and according to our in silico analysis, it seems that albumin fraction contains encrypted peptides that inhibit the dipeptidyl aminopeptidase
IV (DPP-IV) (EC 188.8.131.52) and activate the ubiquitin-mediated proteolysis.
A (APA) is an enzyme that hydrolyzes the N-terminal amino acid of peptides and expressed in both periphery and brain.
Enriched genes in annotation cluster 1 also include aminopeptidases
that hydrolyse antigenic peptides for MHC class I peptide binding and antigen presentation (e.g., endoplasmic reticulum aminopeptidase
ERAP1 and ERAP2), peptide transporter genes (e.g., transported associated with antigen processing, TAP2), and other genes involved in the antigen processing and presentation pathway (e.g., TAP binding protein, TAPBPL; [[beta].sub.2] microglobulin, B2M; CD74).
Kain et al., "Aminopeptidase
N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis," Cancer Research, vol.