In one case, the presence of the SC A-[(1S)-1-(aminocarbonyl)-2-methylpropyl]-1-[(4-fluorophenyl)methyl]-1 H-indazole-3-carboxamide (AB-FUBINACA) was confirmed via HRMS, as published recently (10).
Forty-one out of 42 urine samples were positive for SCs [at least 1 metabolite of UR-144 (1-pentyl-1,ffindol-3-yl)(2,2,3,3-tetramethylcyclopropyl)-methanone), XLR-11 (1-(5-fluoropentyl)-1H-indol-3-yl)(2,2,3,3-tetramethylcyclopropyl)-methanone), AB-CHMINACA (N-[(1S)-1-(aminocarbonyl)-2-methylpropyl]-1(cyclohexylmethyl)-1 H-indazole-3-carboxamide), or ADB-CHMINACA (N-(1-amino-3,3-dimethyl-1-oxobutan-2yl)-1-(cyclohexylmethyl)-1 H-indole-3-carboxamide)], analytically confirmed via LC-MS/MS (see file S3 in the online Data Supplement) (8).
This reaction, called the Maillard or aminocarbonyl
reaction, is accelerated in the state of hyperglycemia in diabetes.
Bolognese et al., "Attenuation of murine collagen-induced arthritis by a novel, potent, selective small molecule inhibitor of IkappaB kinase 2, TPCA-1 (2-[(aminocarbonyl
)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide), occurs via reduction of proinflammatory cytokines and antigen-induced T cell proliferation," Journal of Pharmacology and Experimental Therapeutics, vol.
After starvation at the serum-free medium for 12 hours in the presence or absence of IKK-[beta] inhibitor 2-[(aminocarbonyl)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide (TPCA-1, [IC.sub.50] = 17.9 nM) (Chem Express, China) (40 nM)  and/or TNF-[alpha] (PeproTech, USA) (50 [micro]g/L, 100 [micro]g/L) [13, 26], straight line scratches were created with sterile tips in the cell plate, and then cells were gently washed 3 times by adding serum free L-DMEM medium.
In another study, m-(Diethylaminocarbonyl) benzoic acid (R3N0), m-(ethylaminocarbonyl) benzoic acid (R3N1), and m-(aminocarbonyl
) benzoic acid (R3N2) were found in rat urine (Taylor & Spooner, 1990); and N-ethyl-m-toluamide (R0N1), toluamide (R0N2), N,N-diethyl-m-(hydroxymethyl)benzamide (R1N0), N-ethyl-m-(hydroxymethyl)benzamide (R1N1), and N,N-diethyl-m-formylbenzamide (R2N0) were identified in rat liver microsomes (Taylor, 1986).