aminoacylation


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aminoacylation

The attachment of an amino acid to the acceptor arm of a TRANSFER RNA molecule. This is an essential step in the synthesis of a protein. The attachment is brought about by the appropriate AMINOACYL-TRNA SYNTHETASE.

aminoacylation

a two stage process whereby amino acids are 'activated' in order to be incorporated into proteins; energy in the form of ATP is required and one of a family of 20 aminoacyl-tRNA synthetases, one specific for each amino acid, links the carboxyl group of the amino acid to either the 2′- or 3′-hydroxyl group of the ribose unit at the 3′-end of tRNA.
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Conserved domain, GGCTlike Gamma-glutamyl cyclotransferase (GGCT), the synthesis and metabolism of glutathione 65,420 Dia4p protein-ATP binding, serine- TRNA ligase activity (GO Molecular Function), seryl-tRNA aminoacylation (GO Biological Process) 67,287 Conserved protein Domain family RT_nLTR_like (retrotransposon) 51,829 Conserved protein Domain family RT_nLTR_like (retrotransposon) 73,974 Protein ZDS2; possibly involved in chromatin silencing at rDNA, cell aging, mitotic cell cycling 52,104 Regulation of fatty acid beta-oxidation using acyl-CoA dehydrogenase, mitochondrial respiratory chain complex I assembly 5,799 No description 44,243 Viral capsid protein, controls injection/incorporation of Viral DNA to host.
3243A>G mutation has been shown to lead to reduced levels of the tRNA [sup]Leu (UUR), decrease in aminoacylation, and absence of the normal modification with 5-taurinomethyl group at the wobble base.
Separately, a 3-chlorotyrosinecharged tRNA containing a CUs anticodon, was prepared by a combination of chemical synthesis and enzymatic ligation, followed by aminoacylation of 3-chlorotyrosine using an aminoacyl-tRNA synthetase (Fig.
coli, it has been shown that when the rate of tRNA aminoacylation does not meet the demands of protein synthesis, the ribosome associated RelA is activated (1).
10:00 RIBOZYME-CATALYZED AMINOACYLATION FROM ACETYL COA
Leu(UUR)] mutation causes aminoacylation deficiency and concomitant reduced association of mRNA with ribosomes.
Moreover, it is not difficult to imagine that tRNA aminoacylation activity might also have evolved in the RNA world to regulate RNA replication, perhaps to identify mature genomes in need of replication, or to withdraw genomes from the replicative pool so that they might serve other structural or catalytic functions.
Asn], is also formed by transformation of a mischarged tRNA rather than by direct aminoacylation with asparaginyl-tRNA synthetase.
Species-specific differences in the operational RNA code for aminoacylation of tRNAPro.