Biomedical applications of Limulus amebocyte
To find an easy and efficient way to wipe off the bioburden, we examined three approaches (methods A, B, and C) with different flow rates and flushing durations, using common reagents (75% ethanol, water, and acetic acid), and checked the results using limulus amebocyte
lysate (LAL) clotting test.
Endotoxin levels in the samples were determined using the key quality characteristics limulus amebocyte
Plasma endotoxin concentration was determined by kinetic turbidimetric assay (Yokota et al., 1989), using reagent kit bought from Xiamen tachypleus amebocyte
In order to analyze concentrations of endotoxins and peptidoglycans, frozen filters were warmed up to room temperature and then subsequently eluted with 10 ml of Limulus amebocyte
lysate (LAL) reagent water (Lonza, Ltd., Basel, Switzerland) with 0.05% Tween 20 (Sigma-Aldrich, Ltd., Poznau, Poland) on a platform shaker Tetramax 1000 (Heidolph, Schwabach, Germany) for 15 min, and centrifuged with a force of 1000xg for another 15 min.
A Bacterial Endotoxin Test (BET), also referred to as a limulus amebocyte
lysate (LAL) test, is a biological assay used to detect and quantify the amount of endotoxin and is typically performed after the device is sterilized.
An aliquot (0.1 ml) of stock solution processed above was added to 0.1 ml Limulus amebocyte
lysate (LAL) reagent, and the kinetic turbidity of the mixture was measured using a tube reader (Zhanjiang A and C Biological Ltd., China).
There is a range of tests available for detecting endotoxins including the Rabbit Pyrogen Test (RPT) and tests based on Limulus Amebocyte
Lysate (LAL), which is derived from the blood cells of the horseshoe crab.
(9) The basis of the Fungitell assay relies on BG's ability to activate the limulus amebocyte
lysate clotting cascade present in the blood of Limulus polyphemus, the North American horseshoe crab.
Their blue blood contains cells called Limulus amebocyte
lysate (LAL) that, when used properly, can detect bacteria.
lysate (LAL) assay and in vitro macrophage activation assay
The endotoxin content of these preparations was negligible as determined by the limulus amebocyte
lysate assay (Endos, USA).