amastigotes

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Tambien se investigo la presencia de amastigotes en el liquido sinovial de las articulaciones comprometidas, asi como en el extremo distal de la medula osea de la ultima costilla, para realizar pruebas ante la sospecha de leishmaniasis.
An alternative immunohistochemical method for detecting Leishmania amastigotes in paraffin-embedded canine tissues.
Dogs are considered the main reservoir of amastigotes in urban areas and act as a source of infection for vectors in the domestic environment (MONTEIRO et al., 2005).
Infection was confirmed by identification of Leishmania amastigotes under magnification (100 x).
In her MO aspirate the amastigotes were found, and she died in October 2017.
Amastigotes within the bone marrow smear are diagnostic with 100% specificity but have a sensitivity of only 60-85%.
Los promastigotes se usaron posteriormente para infectar los macrofagos U937 y obtener amastigotes intracelulares (ver abajo).
The precise mechanism of action of intra-lesional glucantime, is not known; the antimony is known to inhibit glycolytic enzymes and fatty acid oxidation in leishmania amastigotes. There is dose dependent inhibition in net formation of adenosine triphosphate [ATP] and guanine triphosphate [GTP]12.
Although Leishmania amastigotes were not observed on the previous bone marrow examination, multiple Leishmania amastigotes and hemophagocytic hystiocytes were observed on bone marrow samples this time (Picture 1).
Los fragmentos fueron Ajados inmediatamente en formol (10%) y embebidos en parafina, para obtener secciones de 3 |j, tenidos con hematoxilina-eosina y monitoreados (1000X) en un doble ciego para cuantificar el numero de seudoquistes contentivos de estadios amastigotes intracelulares en 50 campos, estimandose como: --; +; ++; +++ correspondientes, respectivamente, a parasitismo intracelular ausente; escaso (1-10); moderado (11-20) y abundante (21-30) de acuerdo con Morocoima y col.