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The percentage of infected macrophages and the number of amastigotes per 100 macrophages was determined by counting 100 cells per slide.
Diagnosis of CL was clinically suggested and confirmed by microscope examination of skin scrapings, which revealed typical amastigotes, by a positive Leishmania species-specific PCR result, or both.
Durg susceptibilities of intracellular amastigotes were done with a brief modification that previously described [30], This mixture was incubated at 37[degrees]C in the presence of 5% C[O.
Los frotis y cortes histopatologicos de los hamsteres infectados revelaron amastigotes de L.
Diagnostic investigations in leishmaniasis include gathering suggestive history, examining the patient for typical clinical features, identifying amastigotes by histology or direct microscopy, and growing promastigotes in culture or via polymerase chain reaction (PCR).
Antigenic differences between axenic amastigotes & promastigotes of Leishmania donovani.
Diagnosis of cutaneous leishmaniasis involves unroofing the eschar and taking a scraping of the ulcer base, performing a tissue biopsy to look for amastigotes, or culturing the material for the Leishmania parasite.
Subcellular fractionation, immunohistochemical localization with anti ANT antibodies and surface biotinylation show that the mitochondriai adenine nucleotide translocator is also present in the plasma membrane of both promastigotes and amastigotes [4].
The rupture of the amastigotes causes cellular destruction and an intense inflammatory process, especially in the heart (Cummings and Tarleton, 2004).