A purple nuclear stain used in histology; a mixture of an aqueous solution of ammonium alum and an alcoholic solution of hematoxylin which is ripened or oxidized to hematein.
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Ten micrometers thick tissue sections were cut and placed in distilled water, dipped in alum-hematoxylin solution (for 1-2 min), washed twice with tap water (10 s each), dipped in 1% ammonia solution (for 5-10 s), and then stained with eosin, and dehydrated, cleaned, and mounted.