Western blot analysis for the acetylated
alpha-tubulin levels in these cells demonstrated a reduction in acetyl [alpha]-tubulin expression in lysates of HDAC6 overexpressing HEK293T cells compared to cells transfected with empty vector thus indicating that hdac6 gene cloned by us is catalytically active at protein level (Figure 5(b)).
The microtubular network disorganization and the peculiar location of
alpha-tubulin near the sarcolemma might be responsible for the accumulation of mature autophagic vacuoles observed at 24 h.
Two
alpha-tubulin and two betatublulin isotypes showed preferential accumulation in 10- and 20-dpa fibers, respectively and this accumulation may be correlated with the dramatic changes in cortical microtubule arrays during cotton fiber development (Dixion et al.
Other clones represented non-homeodomain-containing genes, including abundant transcripts like
alpha-tubulin, actin, and collagen.
Milner, "Isotype of
alpha-tubulin are differentially regulated during neuronal maturation," The Journal of Cell Biology, vol.
The nitrocellulose membranes were incubated overnight at 4[degrees]C with antibodies against hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), abhydrolase domain containing protein 5 (ABHD-5), perilipin A, phospho 5' AMP-activated protein kinase (p-AMPK[alpha] 1 e 2 - Thr 172), and
alpha-tubulin obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) diluted 1: 1000 with blocking buffer supplemented with 1% BSA and then washed for 30 min in blocking buffer without BSA.
Alpha-tubulin from early-diverging eukaryotic lineages and the evolution of the tubulin family.
The nitrocellulose membranes were incubated overnight at 4[degrees]C with antibodies against TLR4, myeloid differentiation primary response gene (88) (MyD88), TNF receptor associated factor (TRAF6), hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58 or ABHD5), perilipin A, and
alpha-tubulin obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) diluted 1: 1000 with blocking buffer supplemented with 1% BSA and then washed for 30 min in blocking buffer without BSA.
Alpha-tubulin from early-diverging eukaryotic lineages and evolution of the tubulin family.
The methods for fixing eggs and performing indirect immunofluorescence assays for
alpha-tubulin have been described previously (Gard, 1991; Abraham et al., 1993b).
