PON1, PON3, alpha tubulin
primary antibodies and goat antimouse IgG H&L HRP secondary antibody were purchased from Abcam (Cambridge, UK).
Parte, "HDAC6 deacetylates alpha tubulin
in sperm and modulates sperm motility in Holtzman rat," Cell and Tissue Research, vol.
To visualize hair cells in the squid statocyst, we chose an antibody directed against acetylated alpha tubulin
since acetylation is a common post-translational modification in stable microtubules, which make up kinocilia, and this antigen recognizes alpha tubulin
in many species (Steyger et al., 1989; Perdiz et al., 2011).
The alpha tubulin
(FJ618518), beta tubulin (FJ618519), and actin (FJ618517) genes were used as internal controls (reference genes) to normalize the total RNA amount present in each reaction.
In DMSO-treated myotubes, alpha tubulin
green network runs well-organized and parallel to sarcolemma (Figure 3(a)), while upon 50 [micro]M CisPt alpha tubulin-staining redistributed along a strict peripheral rim or in aggregates at the end of myotubes (Figures 3(b)-3(c)).
David Brown, University of Ottawa); a monoclonal antibody against yeast tubulin clone YOLI/34 (Sera Labs); a monoclonal antibody against native chick brain alpha tubulin
(Amersham); and a monoclonal antibody against Drosophila (insect) beta tubulin, designated E7, which was developed by M.
lophii lends superficial credence to comparative molecular analyses of alpha tubulins
suggesting microsporidia are closely related to fungi (5).