The development of CMS lines possessing different sources of male sterility in the same nuclear background (alloplasmic CMS lines) and crossing these with a single pollen parent will help in better evaluation of cytoplasmic as well as nucleo-cytoplasmic interaction effects.
tuberosus L.) and (ii) cytoplasmic backgrounds including a set of eight HA89 alloplasmic lines (see top of Table 1).
Seven alloplasmic, cytoplasmically male sterile (CMS), HA89 female lines based on the MAX1, PET1, PET2, ANN2, ANN3, GIG1, and PEF1 cytoplasms were crossed with four male parents, HAR4 seln 1, SA52, PAR-1673-2 seln 1, and DES1474-2.
Male sterile (msms) alloplasmic Chris plants carrying the FS2, FS3, FS20, and FS24 mutant genes were crossed to Chris (MsMs) to produce heterozygous male-fertile (Msms) [F.sub.1] plants which were backcrossed to respective male-sterile plants of each mutant.
Because the mutants were induced in alloplasmic Chris, we attempted to avoid any possible epistatic effects by using Chris rather than Chinese Spring (CS) monosomics to determine the chromosomal location of the sterility gene in FS20.
Triticum species differ in regards to compatibility with alien cytoplasms and interspecific nucleocytoplasmic interactions produce a variety of phenotypes, including maternally inherited male sterility, delayed maturity, and reduced plant vigor in the alloplasmic wheat lines (Maan, 1975).