allele-specific PCR

allele-specific PCR

The amplification of specific alleles, or DNA sequence variants, at the same locus by polymerase chain reaction. Specificity is achieved by designing one or both PCR primers so that they partially overlap the site of sequence difference between the amplified alleles.

AS-PCR is two-step nested PCR technique that allows detection of DNA with a point mutation in the presence of a 103–105-fold excess of normal DNA. AS-PCR is based on the observation that Taq polymerase will extend primers with a 3’ mismatch 103 to 106-fold less efficiently than perfectly matched primers; by choosing a primer that is complementary at its 3’ end to a point mutation, selective amplification of a mutant allele can be achieved.
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Keywords: Parkinson's disease, a-Synuclein gene, Pakistani population, Allele-specific PCR, SNCA.
A total of 133 individuals including 60 controls and 73 patients of malaria caused by Plasmodium falciparum were tested for genotype using the allele-specific PCR.
Allele-specific PCR differs from standard real-time PCR in that the primers themselves contain the specific complementary sequence to the relevant area of the EGFR gene, mutation or control.
Allele-specific PCR is a variant form of real-time PCR in which short oligonucleotide probes, specific for the wild-type and variant sequence, are mixed together in the amplification step.
By combining allele-specific PCR with duplex amplification using restricted dNTPs, the copy numbers of SMN1 and SMN2 were clearly identified (Fig.
KRAS mutations were identified in 12 pretreatment specimens, but only 9 posttreatment specimens, despite using laser capture microdissection to increase the proportion of tumor tissues and allele-specific PCR to improve the sensitivity of assays in the latter samples.
LGC's high-throughput genomics services use proprietary sbeadex DNA extraction chemistry and lab-validated genotyping assays, as well as the company's patented KASP fluorescence-based competitive allele-specific PCR platform.
HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays.
Presence or absence of the allele-specific PCR product was used to classify the genotype of the samples (Fig.
An allele-specific PCR assay developed was used to detect sensitive/ resistance alleles among 55 isolates of W.