allele-specific associated primer

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allele-specific associated primer

The sequence of a decamer oligonucleotide used in polymerase chain reaction derived from normal random-amplified polymorphic DNA (RAPD), which generates an absence/presence polymorphism. These absence/presence polymorphisms do not require electrophoretic separation of the sample. Presence of an amplification product is detected by measuring fluorescence of ethidium bromide-stained DNA.
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References in periodicals archive ?
Sequence tagged microsatellite sites (STMS): A total of 7 primers including six sequence tagged microsatellite (STMS) primers and one ASAP (allele specific associated primer) were utilized for the genotyping.
BRIDGING THE CONVENTIONAL AND MOLECULAR METHODS FOR IDENTIFICATION OF RESISTANCE AGAINST FUSARIUM WILT IN CHICKPEA GERMPLASM
Abbreviations: ASAP, allele specific associated primer; ICRISAT, International Crop Research Institute for the Semi-Arid Tropics; PCR, polymerase chain reaction; PDA, potato dextrose agar; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; RIL, recombinant inbred line.
Development of a DNA marker for fusarium wilt resistance in chickpea
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