allele-specific associated primer

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allele-specific associated primer

The sequence of a decamer oligonucleotide used in polymerase chain reaction derived from normal random-amplified polymorphic DNA (RAPD), which generates an absence/presence polymorphism. These absence/presence polymorphisms do not require electrophoretic separation of the sample. Presence of an amplification product is detected by measuring fluorescence of ethidium bromide-stained DNA.
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References in periodicals archive ?
Sequence tagged microsatellite sites (STMS): A total of 7 primers including six sequence tagged microsatellite (STMS) primers and one ASAP (allele specific associated primer) were utilized for the genotyping.
Abbreviations: ASAP, allele specific associated primer; ICRISAT, International Crop Research Institute for the Semi-Arid Tropics; PCR, polymerase chain reaction; PDA, potato dextrose agar; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; RIL, recombinant inbred line.