An increase in leaf ureides (allantoin and allantoic
acid), the major nitrogen compounds exported from soybean nodules, and a simultaneous decrease in nodule activity is a major feature of the drought-stress response.
RNA was extracted from allantoic
fluids using the TRIzol reagent (Life Technologies Cat.
1 mL of the inoculum was injected in the allantoic
cavity of 9-10 day old chicken embryonated eggs and incubated at 37degC for 48-72 hrs.
Identification of IBV by RT-PCR, amplification of S1 gene and cloning in pMD-18T vector: The allantoic
fluid from the dead embryonated eggs showing IBV lesions was collected to identify the virus and to amplify the S1 sub-unit gene.
2 ml of suspension of each concentration of antiviral agent (Lc, Mc, Hc) was introduced through allantoic
route into each of 5 embryonated eggs of subgroups C1, C2 and C3 respectively in order to evaluate drug toxicity.
Fresh infective allantoic
fluid with a HA titer greater than 1/16 (or greater than log24 when expressed as the reciprocal) was diluted 1/10 in sterile isotonic saline.
Viral RNA was obtained from samples of allantoic
fluid using TRIzol LS reagent (Invitrogen, USA).
The virus inoculated via allantoic
sac route disseminates to different parts of embryo via hematogenous route.
Samples that showed a positive reaction in the partial M segment RT-PCR were grown in the allantoic
cavities of 10-day-old specific pathogen-free embryonated chicken eggs.
Concentrations of ureides (allantion and allantoic
acid) in xylem sap were measured as the phenylhydrazine derivative of glyoxylate (Young and Conway.
fluid was harvested 36 h postinfection and tested for HA with chicken erythrocytes to confirm virus isolation (8).
50]) of pooled allantoic
fluid containing A/Anhui/1/2013 (H7N9) in 100 [micro]L of phosphate-buffered saline.