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With alizarin red staining and the use of optical microscopy it was possible to obtain images in all analyzed samples, as well as document and study the morphology of endothelial cells from different corneal regions.
Caption: Figure 1: Oil Red O staining indicating the adipogenesis (red droplets for lipid, (a-d)) and alizarin red staining indicating osteogenesis (red nodules for mineralized calcium, (e-h)).
Calcium nodules stained scarlet with Alizarin red, which are characteristic of osteogenic differentiation, were observed in the MSCs after osteogenic differentiation for 21 days (Figure 1).
The osteogenic differentiation of the BMSCs in the osteogenic media after 21 days was positive and was confirmed by the presence of calcium deposits stained with Alizarin Red (figure 3).
In the case of KAl[(S[O.sub.4]).sub.2] mordant, we found [Al.sup.3+] (not shown), which is usually the only stable not neutral oxidation state of Al in ambient conditions and corroborates the structure of the coordination complex of alizarin and Al proposed in literature [13].
This was visualized by microscopy and quantified spectrophotometrically after staining with oil red O for adipocytes and alizarin red S for osteoblasts (Figures 4(d) and 4(e)).
Cells transfected with agomiR-155 had significantly lower levels of ALP activity (P < 0.01), ALP expression (P < 0.01), and Alizarin Redstained calcification compared to the BMP2 group (P < 0.01).
After three weeks, staining of differentiated caprine En-MSCs in osteogenic media into osteocytes with Alizarin red resulted in presence of calcium deposits and demonstrated mineralized matrix.
The extent of media as well as renal calcification was determined histologically using alizarin red technique.