Multiple aliquots should be created at the beginning of processing a biospecimen rather than delayed until the specific assay is conducted, because repeated freeze-thaw cycles may be detrimental in some cases (e.g., RNA) (7, 86).If the collection site is not close to the laboratory, it may be appropriate to perform simple processes such as on-site centrifugation, aliquoting
, fractionation of serum, and isolation of buffy coat and plasma, and to store the samples in smaller transportable coolers or freezers.
After centrifugation with the Roche Modular Pre-Analytics system (Roche Diagnostics), pipetting error alerts were triggered on the aliquoting
module of the Pre-Analytics system.
The redesign resulted in fewer steps and incorporated one-piece flow to move samples through the accessioning, centrifugation, and aliquoting
According to Taylor, the benefit of this system begins with reduction of draw volume and the avoidance of sample splitting and aliquoting
, where a single tube can be used to analyze > 90% of high-volume tests offered within the core laboratory, such as CBC differential, reticulocyte count, ESR, sickle screen, and Hb Ale.
, the troponin solution was circulated through the pipettor and back into the Teflon bottle to equilibrate the wettable parts of the pipettor with the protein solution.
The Sample Management team sought automation technologies requiring minimal operator intervention for sample acquisition, sample integrity assessment, centrifugation, aliquoting
, stroage, and sample retrieval.
We analyzed the impact of autoverification on the process TAT ("specimen received" time to "result" verified/released time; includes automated accessioning, centrifugation, aliquoting
, and other steps) by comparing statistical data on 4 high-volume tests that were available 24 h/day and orderable as STAT.
For the laboratory scientist engaged in complex processes--such as aliquoting
, precipitation, and purification of samples--the 2D bar code can encode new data at every step of the process and is backward traceable for historical-verification purposes.
We assessed the effects of time and temperature on measured sCD40L concentrations by collecting whole blood from a single healthy individual into a syringe and immediately aliquoting
it into EDTA-containing and plain glass tubes.
Verify that applicable processes have been considered, including the following: collection; aliquoting
; pooling; unit labeling; preparing a new component by washing, irradiation, leukoreduction, etc.; and label ordering.