All the sera were aliquoted
and stored at -20[degrees]C.
We subsequently removed the supernatant and aliquoted
100 [micro]L DMSO into each well to solubilize any resulting formazan crystals.
Using the particle separation and resuspension techniques described above, we developed a 7-step DMF indirect ELISA for RV IgG, in which samples or reagents are aliquoted
to the particles for 4 parallel assays (see online Supplemental Fig.
One mL of the sperm sample was aliquoted
into three parts.
Cells were aliquoted
in 96 well plates (3 x [10.sup.4] cells per well) and activated with indicated concentrations of DNP-conjugated ovalbumin (DNP-Ova) for 30 min.
After centrifugation, 0.3 mL of plasma was aliquoted
from each sample and frozen at -20[degrees]C overnight (~22 hours), and the remaining plasma was stored on cells and analyzed after 1, 3, and 5 hours of storage at room temperature.
A stock solution of clavulanic acid (2000 [micro]g/ml) was prepared, aliquoted
into small vials and stored at -20[degrees]C.
Antibodies can be directly aliquoted
into single 12- x 75-mm sample tubes in 96-tube racks.
According to the company, using the liquid handler, the sample can be aliquoted
and then analyzed with results saved directly in the database, providing efficient operation with advanced security.
For this study, serum specimens from all patients were aliquoted
and stored at -70[degrees]C.
These S-9 (supernatant 9000Xg) preparations were then aliquoted
into five test tubes that contained a prepared reaction mixture that served as a Metabolic Activation System (MAS).
Nontoxic homogenate prepared as described above was aliquoted
into glass vials (5 g meat/vial).