Hydrolysates were derivatized to the alditol
acetates with acetic anhydride-pyridine (Albersheim et al, 1967) and analyzed by CGL using a capillary column Sp 2330 (Supelco) (30x0,25 mm) on a HP-5890 Gas Chromatograph equipped with a flame ionization detector (FID).
The resulting alditol
acetates were analyzed by GC-MS with capillary column DB-225MS (30 m x 0.
Insoluble and soluble non-starch polysaccharides (NSPs) were analyzed by gas chromatography (VARIAN, CP-3800, Walnut Creek, CA, USA) as the alditol
acetate derivatives of monosaccharide based on the method developed by Englyst and Hudson (1993), and Theander and Westerlund (1993).
The results of GCMS analysis of the partially methylated alditol
acetates showed that the EPS consisted of five methylated fragments with different molar ratios (Table 1).
Secondly, it was established that the lipid was alkali stable and on acid hydrolysis yielded two major sugars, tentatively identified by comparative gas chromatography (GC) as the alditol
acetates of the 6-deoxyhexoses, 3,4-di-O-methylrhamnose and 2,3-di-O-methylfucose.
Individual neutral sugars were analyzed as their alditol
acetates by gas-liquid chromatography (GLC) using an HP 5890 SII with FID system, equipped with a capillary column (025 mm [empty set] x 30 m, model B-210, with film 0.
The monosaccharide composition was estimated by gas-chromatography-mass-spectrometry (GC-MS) of alditol
acetate derivates using the reductive hydrolysis procedure (STEVENSON; FURNEAUX, 1991).
21) studied the feasibility of using 2 variants of the alditol
acetate procedure to analyze agar markers associated with spores of Bacillus anthracis.
Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol
14]C-sucrose was incorporated into a trisaccharides group, but these do not contain an alditol
component (BOGO, 2001).
Englyst HN, Wiggins HS, Cummings JH (1982) Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol
acetates of polysaccharide fraction were analyzed by GC/MS (Varian Co.