When analyzing the DEGs at different time points, we found 46, 46, 26, and 24 genes commonly upregulated or downregulated in the pathways of agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis, IL-10 signaling, and IL-6 signaling, respectively (see the intersect in red, Figures 2(a), 2(b), 2(c), and 2(d)).
Signaling pathway analysis suggested that agranulocyte adhesion and diapedesis, granulocyte adhesion and diapedesis pathway, IL-6 signaling, and IL-10 signaling are the major immune/inflammation pathways upregulated during nerve degeneration and regeneration.
Caption: Figure 2: Differential gene expression patterns in the pathways of agranulocyte adhesion and diapedesis (a), granulocyte adhesion and diapedesis (b), IL-6 signaling (c), and IL-10 signaling (d) at 1, 4, 7, and 14 days after injury shown as Venn diagrams.
On Hemacolor smears, 2 hemocyte types were distinguished by light microscopy: granulocytes and hyalinocytes (or agranulocytes) according to the presence or the absence of cytoplasmic granules.
Cheng (1981) presented a morphological scheme based on numbers of cytoplasmic granules, dividing cells into 2 types: granulocytes, cells containing granules that ranged from very few to numerous; and agranulocytes, cells containing few or no granules.
After the separation in this type of density gradient, the granulocytes were separated from agranulocytes and pure granulocytes were obtained.