Gmelin) Howe and its cinnamic acids was studied in GT1-7 cells exposed to the combination of chemical hypoxia (KCN 3 mM) and aglycemia conditions.
Keywords: Bryothamnion triquetrum; GT1-7 cell line; Chemical hypoxia; Aglycemia; Antioxidant; Seaweed
In neural cultures, a combination of aglycemia conditions and chemical hypoxia induced by cyanide are used to mimic the rapid oxygen and glucose deprivation that occurs in a brain undergoing ischemia (Rego et al., 1999).
Thus, the aim of the present study was to evaluate the neuroprotective and antioxidant properties of the aqueous extract of Bt and its cinnamic acids in GT1-7 cells exposed to chemical hypoxia and aglycemia, by causing an insult that mimics some of the ischemic events.
Additionally, the effects of separated hypoxia and aglycemia insults on cell viability were assessed in the same way at similar time-points.
The immediate GT1-7 cell viability measured after the exposure to separated chemical hypoxia (91[+ or -]5) or aglycemia (100[+ or -]0) during 180 min was not statistically different from the survival of control cells (97[+ or -]1).
Data from Table 2 show that glucose exerted significant protection against severe chemical hypoxia/strict aglycemia conditions only at concentrations over 500 [micro]M.