Glucosamine also inhibited the aggrecan
degradation in a rat chondrosarcoma cell line and bovine cartilage explants, a result that was mediated by aggrecanase, a proteinase induced by interleukin-1 or retinoic acid.
IBEX expects to follow the release of new fetal aggrecan
assay with the release later this year of two additional next-generation arthritis assays, CPII and C2C, for use by the pharmaceutical community.
SB-061, which will be administered as an intra-articular injection, is designed to prevent the degradation of cartilage by mimicking the protective effect of the proteoglycan aggrecan
The breakdown of aggrecan
, in particular, which is included in the extracellular matrix, is critical in articular damage.
Researchers in Grodzinsky's lab are now working to identify possible drugs that might halt the loss of aggrecan
, as well as designing tissue scaffolds that could be implanted into patients who need cartilage-replacement surgery.
Primers for differentiation markers (Sox9, Aggrecan
precursor, Collagen type 2 alpha (COL2A)) and GAPDH internal control were shown in Table 2.
INC/ Name: Collagen Peptide Aggrecan
Explant and monolayer cell culture data differed in the amount of gene activity observed, especially for aggrecan
and Collagen Type X, but MMP-13 was reduced by vitamin C at both normoxic and hypoxic levels.
FGF-2 activates a compound called Sox9 in the stem cell, which in turn switches on the production of two main components of cartilage, type 2 collagen and aggrecan
, Li says.
In these cells, it is noteworthy that aggrecan
and type X collagen are constitutively expressed.
Candidate polysaccharide or proteoglycan subunits include aggrecan
, agrin, bamacan, heparan sulfate, chondroitin sulfate, keratan sulfate, versican, phosphocan, perlecan, hyaluronan, decorin, dermatan sulfate, biglycan, fibromodulin, alginate, polylactate, polyglycolic acid, starch, dextran, agarose, chitan, chitosan, and heparin.
HP appears to modulate aggrecan
biosynthesis through membrane-mediated pathways, such as the transport of cations, aminoacids and macromolecules.