agarose gel electrophoresis


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Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
Second, the agarose gel electrophoresis requires that students analyze their gels carefully.
Agarose gel electrophoresis of PCR product for 16S rRNA amplified gene (size more than 1.4 Kb) compared with (1Kb) DNA ladder
Caption: Agarose Gel electrophoresis of PCR amplified 16S 23S rDNA inter transcribed spacer (ITS) region (110 bp) of K.
Agarose gel electrophoresis of plasma DNA obtained from samples after HD revealed the typical apoptotic ladders.
Evaluation of alkaline phosphatase isoenrymes in sera of hemodialysis and periton dialysis patients by agarose gel electrophoresis. Biochem Soc Trans 1995;23:310S.
The Xyn 2 gene is ligated successfully with pUC19 by T4 DNA ligase enzyme and verified with Agarose gel electrophoresis (Fig 2).
Serum and urine agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE) were performed with, respectively, Paragon SPE Gel and Paragon Twin IFE Gel reagents (both from Beckman Coulter).
The amplified PCR product was separated by agarose gel electrophoresis visualized by ethidium bromide staining and sequenced directly on an ABI PRISM 3100 Genetic Analyzer with ABI PRISM BigDye Terminator v3.0 sequencing kit (Applied Biosystems, Foster City, CA).
Agarose gel electrophoresis was performed to resolve the amplification product using 1.2 per cent agarose in 1X TBE buffer, 0.5[micro]g/ml of Ethidium bromide, and loading buffer (0.25% Bromophenol blue in 40% sucrose).
This relatively rapid clearance suggested a nonprotein compound, which was confirmed by the absence of an abnormal band on agarose gel electrophoresis (AGE; Paragon[R] SPE Kit; Beckman Coulter).