agarose gel electrophoresis


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Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
The gDNA extracted from human blood by alkali lysis method, as visualized in Agarose gel electrophoresis after Ethidium bromide staining is shown in Fig.
Because of the large number of small fragments generated for all six of the species by Mnl I, this enzyme was excluded from further analysis, because differences in the interspecific patterns would have been difficult to resolve using agarose gel electrophoresis.
Agarose gel electrophoresis as described here is simple, cheap, and effective, and it remains a useful tool in research and teaching labs, but it is a poor method now rarely used in clinical lab settings.
Module 3: Agarose gel electrophoresis of PCR products
4-Qualitative Plasmid DNA control by agarose gel electrophoresis
In both samples the CZE electropherograms showed a minor disturbance in morphology of the [gamma]-region but not the obvious monoclonal peak that was observed with agarose gel electrophoresis.
Subsequently, 10 [micro]L of the amplified products was analyzed by using 2% agarose gel electrophoresis.
We used agarose gel electrophoresis to analyze the fragmentation of DNA during apoptosis.
We are using eGene's automated HDA-GT12(TM) genetic analyzer that replaces our current manual agarose gel electrophoresis process for high-resolution HLA SSP (sequence specific primer) genotyping," said Dr.
Sebia Electrophoresis provides multiple platforms for the detection of hemoglobinopathies and thalassemias--fully automated capillary electrophoresis systems and a semi-automated agarose gel system to accommodate both alkaline and acid agarose gel electrophoresis.