agarose


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ag·a·rose

(ag'ă-rōs),
The neutral linear polysaccharide fraction found in agar preparations, generally composed of d-galactose and altered 3,6-anhydro-l- galactose residues; used in chromatography and electrophoresis.

agarose

(ä′gə-rōs′, -rōz′, ăg′ə-)
n.
A polysaccharide obtained from agar that is the most widely used medium for gel electrophoresis procedures.

agarose

[ag′ärōs]
an essentially neutral fraction of agar used as a medium in electrophoresis, particularly for separation of serum proteins, hemoglobin variants, and lipoprotein fractions.

Agarose

Chemical A linear galactan obtained from purified agar which is used as a support (gel) for many types of electrophoresis and immunodiffusion; a typical gel contains ±1% agarose.
Molecular biology An uncharged polysaccharide (carbohydrate polymer) purified from agar which melts when heated to 100ºC and resolidifies when cooled below about 45°C, forming a matrix, which can be used for agarose gel electrophoresis.

ag·a·rose

(ag'ă-rōs)
The neutral linear polysaccharide fraction found in agar preparations; used in chromatography and electrophoresis.

agarose

more highly purified form of agar with similar uses to agar and widely used in the separation of nucleic acid fragments.
References in periodicals archive ?
Masson's trichrome staining of the tissue-engineered vesical equivalents treated with paper and agarose vectors for the 0.
59 agarose corrected for dilutiond Ortho Vitros ECi Untreated 1.
Let's stop and consider what this process has done for us, compared to agarose gel endpoint PCR analysis:
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining.
It can be assumed that agarose gel is almost incompressible (Poisson ratio v [approximately equal to] 1/2) [12] and that a plane-stress condition, and monotonic loading exist in the J-integral test [26]; thus, the deformation theory of plasticity was invoked as [27]:
Finally, we electrophoresed LightCycler PCR products on a 20-g/L agarose gel stained with ethidium bromide to confirm the presence of the 115-, 247-, and 150-bp amplicons for the ALU115, ALU247, and CDKN2A sequences, respectively.
495]) eNOS, mouse anti-phosphotyrosine (PY99), rabbit anti-eNOS (NOS3, N-20), rabbit anti-p-Akt1(serine at codon 473), goat anti-human Akt, protein A agarose, and secondary antibodies (both horseradish peroxidase-conjugated and fluorescein-conjugated) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
In a test performed by BIPHA Corporation, a subsidiary of Yoshitomi Pharmaceutical Industries in Japan and an Amersham customer, STREAMLINE SP adsorbents, a mixture of agarose and crystalline quartz, proved remarkably reliable, lasting more than 1,000 cycles without a dip in performance.
UltraClean 15 utilizes a silica binding particle method to purify DNA fragments from TAE or TBE agarose gels.
In the course of this troublesome study series, which involved purely chemical methods, a unique component sugar, 3,6-anhydrogalactose, was discovered [4] and an idealized extreme structure type of agarose (Fig.