agarose


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ag·a·rose

(ag'ă-rōs),
The neutral linear polysaccharide fraction found in agar preparations, generally composed of d-galactose and altered 3,6-anhydro-l- galactose residues; used in chromatography and electrophoresis.

agarose

(ä′gə-rōs′, -rōz′, ăg′ə-)
n.
A polysaccharide obtained from agar that is the most widely used medium for gel electrophoresis procedures.

Agarose

Chemical A linear galactan obtained from purified agar which is used as a support (gel) for many types of electrophoresis and immunodiffusion; a typical gel contains ±1% agarose.
Molecular biology An uncharged polysaccharide (carbohydrate polymer) purified from agar which melts when heated to 100ºC and resolidifies when cooled below about 45°C, forming a matrix, which can be used for agarose gel electrophoresis.

ag·a·rose

(ag'ă-rōs)
The neutral linear polysaccharide fraction found in agar preparations; used in chromatography and electrophoresis.
References in periodicals archive ?
SEM analysis of the agarose on the glass slides showed a porous film (Figure 4).
The gDNA extracted from human blood by alkali lysis method, as visualized in Agarose gel electrophoresis after Ethidium bromide staining is shown in Fig.1.
It can be assumed that agarose gel is almost incompressible (Poisson ratio v [approximately equal to] 1/2) [12] and that a plane-stress condition, and monotonic loading exist in the J-integral test [26]; thus, the deformation theory of plasticity was invoked as [27]:
When cDNA was synthesized using 2 ug RNA and RT-PCR was carried out, faint bands of the samples were observed on agarose gel, which is indicative of the inadequate amount of viral RNA.
Two molecular beacons, modified at the 5'-terminal with N[H.sub.2] groups and immobilized on activated agarose film, were used for HCoV-SARS detection, as shown in Table 3 in the online Data Supplement).
The whole cycle of investigations concerning algal polysaccharides was introduced by elucidation of promising seaweed species containing a nearly neutral galactan fraction close to agarose (Fig.
Embryos fertilized in vitro may be successfully cultured on cushions of 0.2% agarose in Millipore (0.22 [micro]m) filtered seawater (MFSW) (Sigma, Type II) (7), but their chorions do not reliably swell to permit complete development or hatching.
Various techniques, including agarose gel electrophoresis or DNA hybridization with calorimetric or chemiluminescent detection, can detect the PCR products.
Step three was to run an agarose gel electrophoresis.
The hypocotyl showed more DNA agarose bands than hypocotyl groups under drought stress.
Tardis - an acronym for "trapped in agarose DNA immunostaining" - allows doctors to get an inside view of cancer cells and see if the drugs used to attack them are working.