Micromonospora in
aerial mycelium white color and substrate mycelium pink color were formed in PDA media.
In order study the morphological characteristics with reference to aerial mycelium, substrate mycelium and sporulation, the isolated colonies were cultivated on glycerol asparagines agar by simple "Inclined cover slip culture technique" (Gibbs & shapton, 1968) as detailed below.
Then coverslip was carefully withdrawn from the medium and observed directly under microscope to differentiate between substrate and aerial mycelium.
On PDA the colonies produced cottony aerial mycelium that was grayish-white at first, later becoming cream-colored to light brown.
The colonies were floccose and the surface mycelium was violet while the aerial mycelium was yellowish brown; the colony reverse was dark,
Ascomata were non-ostiolate, superficial, white to light cream colored, globose or subglobose, measuring 120 [micro]m-600 [micro]m in diameter, and covered with a white
aerial mycelium.
After incubation at 30 [degrees] C for 7 days, the colonies demonstrated gray
aerial mycelium.
No barrage or flat reactions were noted, although some mating exhibited lethal reactions on
AERIAL mycelium, not agar-surface.
The surface of colony was loose with intensive radial lines; the
aerial mycelium in the middle of colony has projections; the circle of colony was hair like.
5 mm in diameter) in culture medium (PDA), were incubated at room temperature for 7 days, the growth rate was measured every 24 h until the completion of the total colonization of the strain, the macroscopic morphological characteristics of the colonies were recorded regard to texture, density,
aerial mycelium and color.
The observation was on
aerial mycelium, the colour of substratum, texture, zonation until the isolate covered the plate.
Mature
aerial mycelium and substrate mycelium pigmentation were recorded on ISP5, aerial mass color on ISP3 and ISP4, melanin production on ISP6 and ISP7 following incubation at 27oC for 21 days using a reference color key (Prauser, 1964).
