technology, based on a naturally occurring photoprotein, generates large, luminescent signals that result in higher quality data, while reducing the number of false positives in high throughput and ultra-high throughput screening environments.
glows blue when calcium ions bind to it, but it's more than just a pretty protein.
"One of the reasons that we're pursuing a modified Tetra is that we find that the current suppliers of readers for aequorin
don't have the preferred features that our customers want.
This discovery led to the use of aequorin
as an important biomedical tool for tracking the movement of calcium within cells.
The observations may also be relevant in explaining the efficient energy transfer between aequorin
and GFP in the light-emitting organ of the jellyfish itself.
The output of this model (mentioned above) evokes a calcium signal detectable with high sensitivity aequorin
(e.g., 4-6) within 250 [micro]s of the start of [PLA.sub.2] activity, assuming a single calcium ion is released per [LtB.sub.4] produced.
In the class Hydrozoa, all reported cases of luminescence are caused by [Ca.sup.2+]-sensitive photoproteins such as aequorin
, found in the jellyfish Aequorea aequorea (Shimomura et al., 1962), and obelin, obtained from the hydroid Obelia sp.
preparations (18) were generously provided by Dr.
preparations (24) were generously provided by Dr.
The blastomeres were injected with 10-pl doses of semisynthetic aequorin
(17-19) shortly after the first cytokinesis following fertilization.
Extraction of Renilla-type luciferin from the calcium-activated photoproteins aequorin
, mnemiopsin and berovin.
Individual calcium release events were visualized as bright observable blobs in aequorin
labeled cells (6, 7) and they occur within restricted regions of space called microdomains (6, 7).