A part of aequorin
called coelenterazine reacts with oxygen to produce coelenteramide and carbon dioxide, while emitting light.
assays are a sensitive and flexible cell-based assay technology used to detect GPCR activation with several advantages over conventional fluorescence based dyes, including fewer false positives, much simpler protocol and significantly increased assay windows.
Once the ability of aequorin
to protect brain cells from death was proven and the research results were published and presented at national scientific conferences, Underwood and his team at Quincy Biosciences concentrated their efforts on the manufacturing and launch of Prevagen as a dietary supplement.
This discovery led to the use of aequorin
as an important biomedical tool for tracking the movement of calcium within cells.
was first isolated from simple marine jellyfish in the 1960s and then identified as a member of a family of proteins that binds to calcium and influences cell functions throughout the body.
Studies conducted by Quincy Bioscience in conjunction with scientists at the University of Wisconsin-Milwaukee have shown that the jellyfish protein aequorin
results in a significant reduction of brain cell death in their animal studies.
The bioluminescent jellyfish Aequorea emits 'green' light in vivo, whereas the pure photoprotein aequorin
extracted from the same organism emits 'blue' light on addition of [Ca.
The observations may also be relevant in explaining the efficient energy transfer between aequorin
and GFP in the light-emitting organ of the jellyfish itself.
The output of this model (mentioned above) evokes a calcium signal detectable with high sensitivity aequorin
Technology Would Strengthen Leadership Position in GPCR Screening
2+]-sensitive photoproteins such as aequorin
, found in the jellyfish Aequorea aequorea (Shimomura et al.
preparations (18) were generously provided by Dr.