The in vitro differentiation of MSCs into adipogenic
differentiation was seen as early as 7 days of induction in adipogenic
differentiation medium, the cells start showing the presence of lipid vacuoles under the microscope.
Oil red staining was conducted to show lipid vesicles to confirm the adipogenic
Oil red O staining could be used to identify adipogenic
differentiation of the AMSCs.
After three days of expansion until confluency, adipogenic
differentiation was initiated through the addition of defined adipogenic
differentiation medium as described before.
In addition, the role of adiponectin in the adipogenic
differentiation of muscle satellite cells is unknown.
MenSCs and BMSCs were differentiated into adipogenic
, chondrogenic and osteogenic lineages using specific polarizing media as per method described previously (24,25).
When cells reached 80-90% confluency, media were replaced with differentiation media [Alpha Modification of Eagle's Medium (aMEM), 15% FBS supplemented with adipogenic
induction cocktail (MDI: 500 micromolar isobutylmethylxanthine, 1 micromolar dexamethasone, 5 microgram/mL human recombinant insulin and ligands].
genes CEBPa and FABP4 were up-regulated in the sucralose-exposed MSCs, which also showed more intracellular fat droplet accumulation.
Characterizations of MSCs were also tested by osteogenic and adipogenic
differentiation and MSCs-related marker staining, as described in our previous study.
Renevia's hydrogel polymer network provides the requisite amino acid sequences for adipose stromal vascular cell attachment and may support proliferation, localization, and adipogenic
Differentiation of fibroblastic cells obtained from adipose tissue could be directed towards adipogenic
, chondrogenic and osteogenic lineages using appropriate differentiation media and supplements.
We chose to divide the sections into adipogenic
and nonadipogenic cells inspired by the paper of Yang et al., who proposed and proved different seipin functions for these two models .