Adenylates generally can only account for a very short time supply of energy.
For the determination of the initial adenylate levels, clams were allowed to recover from possible collection stress for one week under aquarium conditions before they were sampled.
The neutralized supernatants were analyzed for adenylates by high-performance liquid chromatography using weak anion exchange as previously described (Rees and Hand, 1991).
Finally, we measured ATP, ADP, and AMP in post-diapause gemmules to gain insight into the adenylate status during development.
The calculated adenylate energy charge was statistically unchanged over the 54 h of aerobic incubation (Table I).
Table I ATP:ADP ratios and adenylate energy charge in gemmules of Eunapius fragilis during germination Time at room temperature (h) ATP:ADP ratio(a) Energy charge(a) 0 6.91 [+ or -] 0.96 0.81 [+ or -] 0.02 6 15.12 [+ or -] 1.31 0.86 [+ or -] 0.01 18 12.15 [+ or -] 1.89 0.83 [+ or -] 0.01 30 10.28 [+ or -] 0.05 0.80 [+ or -] 0.01 42 17.55 [+ or -] 0.92(*) 0.79 [+ or -] 0.01 54 9.86 [+ or -] 2.57 0.77 [+ or -] 0.05 a Values represent means [+ or -] 1 SE (n = 3).